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Literature summary extracted from

  • Moche, M.; Dehesh, K.; Edwards, P.; Lindqvist, Y.
    The crystal structure of beta-ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54.ANG. resolution and its relationship to other condensing enzymes (2001), J. Mol. Biol., 305, 491-503.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.41 medicine target for the development of drugs for the treatment of cancer and tuberculosis Synechocystis sp.
2.3.1.41 nutrition target for the engineering of plant seed oils Synechocystis sp.
2.3.1.41 pharmacology target for the development of drugs for the treatment of cancer and tuberculosis, involved in biosynthesis of precursors of pharmacological agents Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 expression in Escherichia coli Synechocystis sp.
2.3.1.179 expression in Escherichia coli Synechocystis sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.41 hanging drop vapour diffusion method, protein solution: 25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 75 mM imidazole, reservoir solution: 0.085 M tri-sodium citrate, 0.17 M ammonium acetate, 22% w/v polyethylene glycol 4000, 15% v/v glycerol as cryo-protectant, 15% w/v 1,2,3-heptanetriol, x-ray structure analysis, three-dimensional model Synechocystis sp.
2.3.1.179 rod-shaped crystals of the purified enzyme are grown in two weeks by the hanging-drop vapor-diffusion method, 1.54 A resolution, space group P3(1)21, cells dimensions: a = b = 100.8 A, c = 74.7 A Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Synechocystis sp.
-
 isozyme II
-
2.3.1.179 Synechocystis sp. P73283 PCC 6803
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] structure of active site, substrate binding pocket, and subunit structure in relation to substrate specificity of the 3 isozyme types Synechocystis sp.
a
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] active site cysteine Synechocystis sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Synechocystis sp. 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.41 dimer crystal structure analysis of subunits Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 KAS
-
 Synechocystis sp.
2.3.1.41 More 3 structural classes of condensing enzymes Synechocystis sp.