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Literature summary extracted from

  • Smirnova, N.; Reynolds, K.A.
    Engineered fatty acid biosynthesis in Streptomyces by altered catalytic function of beta-ketoacyl-acyl carrier protein synthase III (2001), J. Bacteriol., 183, 2335-2342.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 plasmid-based expression of synthase III wild-type and mutants in Streptomyces glaucescens and His-tagged in Escherichia coli Streptomyces glaucescens
2.3.1.180 subcloning in Escherichia coli strain TG-2, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces glaucescens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.41 C122A site-directed mutagenesis, 75% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type Streptomyces glaucescens
2.3.1.41 C122Q site-directed mutagenesis, 500% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type Streptomyces glaucescens
2.3.1.41 C122S site-directed mutagenesis, enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type Streptomyces glaucescens
2.3.1.180 C122A site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 75% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions Streptomyces glaucescens
2.3.1.180 C122Q site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 500% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions Streptomyces glaucescens
2.3.1.180 C122S site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 100% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions Streptomyces glaucescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 butyryl-CoA synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of Streptomyces glaucescens
2.3.1.41 isobutyryl-CoA synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Streptomyces glaucescens initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens the enzyme initiates the fatty acid biosynthesis, overview acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Streptomyces glaucescens
-
 synthase III, gene fabH
-
2.3.1.180 Streptomyces glaucescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 recombinant from E. coli as His-tagged protein Streptomyces glaucescens
2.3.1.41 wild-type and mutants Streptomyces glaucescens
2.3.1.180 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces glaucescens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] active site cysteine Streptomyces glaucescens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 isobutyryl-CoA + malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens ?
-
 ?
2.3.1.41 isobutyryl-CoA + malonyl-[acyl-carrier protein] isobutyryl-CoA is a precursor of branched-chain fatty acid synthesis Streptomyces glaucescens ?
-
 ?
2.3.1.41 malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens acetyl-[acyl-carrier protein] + CO2
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III, broad specificity Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] the enzyme initiates the fatty acid biosynthesis, overview Streptomyces glaucescens acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 KAS
-
 Streptomyces glaucescens
2.3.1.180 ACO synthase III
-
 Streptomyces glaucescens
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Streptomyces glaucescens
2.3.1.180 KASIII
-
 Streptomyces glaucescens