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Literature summary extracted from

  • Val, D.; Banu, G.; Seshadri, K.; Lindqvist, Y.; Dehesh, K.
    Re-engineering ketoacyl synthase specificity (2000), Structure, 8, 565-566.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.41 medicine target for the development of drugs for the treatment of cancer and tuberculosis Synechocystis sp.
2.3.1.41 nutrition target for the engineering of plant seed oils Synechocystis sp.
2.3.1.41 pharmacology target for the development of drugs for the treatment of cancer and tuberculosis, involved in biosynthesis of precursors of pharmacological agents Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 expression of wild-type and mutant synthase II in Escherichia coli Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.41 A193M site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 2.55fold, 12fold reduced catalytic activity Synechocystis sp.
2.3.1.41 I108F site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 1.75fold, 38fold reduced catalytic activity towards octanoyl-[acyl-carrier-protein] compared to the activity towards hexanoy-[acyl-carrier-protein] Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 cerulenin 12-carbon mycotoxin, irreversible inhibition, binding motif; at 0.05 mM: wild-type is completely inactivated, mutant I108F and A193M show only 10% reduced activity Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Synechocystis sp.
-
 synthase II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 synthase II, recombinant from E. coli Synechocystis sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] active site cysteine Synechocystis sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Synechocystis sp. 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Synechocystis sp. 3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Synechocystis sp. 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 KAS
-
 Synechocystis sp.