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Literature summary extracted from

  • Bao, W.; Sheldon, P.J.; Hutchinson, C.R.
    Purification and properties of the Streptomyces peucetius DpsC beta-ketoacyl:acyl carrier protein synthase III that specifies the propionate-starter unit for type II polyketide biosynthesis (1999), Biochemistry, 38, 9752-9757.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 expression of synthase III-like polyketid synthase in Streptomyces lividans Streptomyces peucetius
2.3.1.180 expression in Streptomyces lividans Streptomyces peucetius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 38000
-
synthase III-like polyketid synthase, gel filtration, amino acid sequence determination Streptomyces peucetius
2.3.1.180 37189
-
1 * 37189, recombinant enzyme, mass spectrometry Streptomyces peucetius
2.3.1.180 38000
-
gel filtration, recombinant enzyme Streptomyces peucetius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 propionyl-CoA + malonyl-[acyl-carrier protein] Streptomyces peucetius the enzyme specifies the propionate-starter unit for type II polyketide biosynthesis 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Streptomyces peucetius
-
synthase III-like activity
-
2.3.1.180 Streptomyces peucetius
-
gene dpsC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 synthase III-like polyketid synthase recombinant from Streptomyces lividans Streptomyces peucetius
2.3.1.180 recombinant enzyme from Streptomyces lividans by ammonium sulfate fractionation, gel filtration, ion exchange and hydrophobic interaction chromatography Streptomyces peucetius

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] acetylation of the synthase III-like polyketid synthase at Ser118 Streptomyces peucetius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] absolutely specific for Streptomyces peucetius beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.180 additional information no activity with acetyl-CoA, malonyl-CoA, and 2-methylmalonyl-CoA as primer for elongation of fatty acids, the enzyme shows also acyl-CoA:ACP transacylase activity for transfer of propionate to ACP Streptomyces peucetius ?
-
?
2.3.1.180 propionyl-CoA + malonyl-[acyl-carrier protein] the enzyme specifies the propionate-starter unit for type II polyketide biosynthesis Streptomyces peucetius 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 propionyl-CoA + malonyl-[acyl-carrier protein] intermediate binding of propionyl to the enzyme at Ser118 Streptomyces peucetius 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 monomer 1 * 38000, synthase III-like polyketid synthase, SDS-PAGE Streptomyces peucetius
2.3.1.180 monomer 1 * 37189, recombinant enzyme, mass spectrometry Streptomyces peucetius

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 DpsC
-
Streptomyces peucetius
2.3.1.41 More gene DpsC, encoding apolyketide synthase, exhibits also beta-keto-[acyl-carrier-protein] synthase III activity, which is restricted for propionyl-[acyl-carrier-protein] as the only [acyl-carrier-protein]-substrate Streptomyces peucetius
2.3.1.180 beta-ketoacyl:acyl carrier protein synthase III
-
Streptomyces peucetius
2.3.1.180 DpsC
-
Streptomyces peucetius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 22
-
assay at room temperature Streptomyces peucetius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7.2
-
assay at Streptomyces peucetius