EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.41 | expression of synthase III-like polyketid synthase in Streptomyces lividans | Streptomyces peucetius |
2.3.1.180 | expression in Streptomyces lividans | Streptomyces peucetius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.41 | 38000 | - |
synthase III-like polyketid synthase, gel filtration, amino acid sequence determination | Streptomyces peucetius |
2.3.1.180 | 37189 | - |
1 * 37189, recombinant enzyme, mass spectrometry | Streptomyces peucetius |
2.3.1.180 | 38000 | - |
gel filtration, recombinant enzyme | Streptomyces peucetius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.180 | propionyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces peucetius | the enzyme specifies the propionate-starter unit for type II polyketide biosynthesis | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.41 | Streptomyces peucetius | - |
synthase III-like activity | - |
2.3.1.180 | Streptomyces peucetius | - |
gene dpsC | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.41 | synthase III-like polyketid synthase recombinant from Streptomyces lividans | Streptomyces peucetius |
2.3.1.180 | recombinant enzyme from Streptomyces lividans by ammonium sulfate fractionation, gel filtration, ion exchange and hydrophobic interaction chromatography | Streptomyces peucetius |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | acetylation of the synthase III-like polyketid synthase at Ser118 | Streptomyces peucetius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.41 | propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | absolutely specific for | Streptomyces peucetius | beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.180 | additional information | no activity with acetyl-CoA, malonyl-CoA, and 2-methylmalonyl-CoA as primer for elongation of fatty acids, the enzyme shows also acyl-CoA:ACP transacylase activity for transfer of propionate to ACP | Streptomyces peucetius | ? | - |
? | |
2.3.1.180 | propionyl-CoA + malonyl-[acyl-carrier protein] | the enzyme specifies the propionate-starter unit for type II polyketide biosynthesis | Streptomyces peucetius | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | propionyl-CoA + malonyl-[acyl-carrier protein] | intermediate binding of propionyl to the enzyme at Ser118 | Streptomyces peucetius | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.41 | monomer | 1 * 38000, synthase III-like polyketid synthase, SDS-PAGE | Streptomyces peucetius |
2.3.1.180 | monomer | 1 * 37189, recombinant enzyme, mass spectrometry | Streptomyces peucetius |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.41 | DpsC | - |
Streptomyces peucetius |
2.3.1.41 | More | gene DpsC, encoding apolyketide synthase, exhibits also beta-keto-[acyl-carrier-protein] synthase III activity, which is restricted for propionyl-[acyl-carrier-protein] as the only [acyl-carrier-protein]-substrate | Streptomyces peucetius |
2.3.1.180 | beta-ketoacyl:acyl carrier protein synthase III | - |
Streptomyces peucetius |
2.3.1.180 | DpsC | - |
Streptomyces peucetius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.180 | 22 | - |
assay at room temperature | Streptomyces peucetius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.180 | 7.2 | - |
assay at | Streptomyces peucetius |