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Literature summary extracted from

  • Garwin, J.L.; Klages, A.L.; Cronan, J.E.
    Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli (1980), J. Biol. Chem., 255, 11949-11956.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.41 dithiothreitol required Escherichia coli
2.3.1.41 dithiothreitol activation Escherichia coli
2.3.1.41 EDTA activation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 additional information
-
 additional information kinetic data of synthase I and II at different temperatures with various substrates Escherichia coli
2.3.1.179 0.024
-
 dodec-5-enoyl-[acyl-carrier protein] 37°C Escherichia coli
2.3.1.179 0.024
-
 dodec-5-enoyl-[acyl-carrier protein] 27°C Escherichia coli
2.3.1.179 0.043
-
 tetradec-7-enoyl-[acyl-carrier protein] 27°C Escherichia coli
2.3.1.179 0.047
-
 tetradecanoyl-[acyl-carrier protein] 27°C Escherichia coli
2.3.1.179 0.06
-
 tetradec-7-enoyl-[acyl-carrier protein] 37°C Escherichia coli
2.3.1.179 0.068
-
 tetradecanoyl-[acyl-carrier protein] 37°C Escherichia coli
2.3.1.179 0.097
-
 palmitoleoyl-[acyl-carrier protein] 27°C Escherichia coli
2.3.1.179 0.216
-
 palmitoleoyl-[acyl-carrier protein] 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 additional information
-
 amino acid composition, synthase I and II Escherichia coli
2.3.1.41 80000
-
 synthase I, sedimentation equilibrium method Escherichia coli
2.3.1.41 85000
-
 synthase II, sedimentation equilibrium method Escherichia coli
2.3.1.179 85000
-
 equilibrium sedimentation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.179 additional information Escherichia coli proposed role of the enzyme in the modulation of fatty acid synthesis by temperature ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Escherichia coli
-
 synthases I and II
-
2.3.1.41 Escherichia coli
-
 K12 strains UC1, WN1 is a fabF-mutant lacking synthase II, and various mutants, overview
-
2.3.1.179 Escherichia coli P0AAI5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 synthases I and II Escherichia coli
2.3.1.179
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] structure Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 5.5
-
 synthase I Escherichia coli
2.3.1.41 6.3
-
 synthase II Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] poor substrate, synthase I Escherichia coli 3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] best substrate, synthase II Escherichia coli 3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] not cis-vaccenoyl-[acyl-carrier-protein], i.e. cis-11-octenoyl-[acyl-carrier-protein] Escherichia coli cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates Escherichia coli cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] i.e. palmitoyl-[acyl-carrier-protein] Escherichia coli 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.179 dodec-5-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli ? + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.179 additional information proposed role of the enzyme in the modulation of fatty acid synthesis by temperature Escherichia coli ?
-
 ?
2.3.1.179 palmitoleoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli cis-vaccenoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.179 tetradec-7-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli ? + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.179 tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli ? + CO2 + [acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 dimer 2 * 43000-44000, synthase I, SDS-PAGE Escherichia coli
2.3.1.41 dimer 2 * 44000-45000, synthase II, SDS-PAGE Escherichia coli
2.3.1.179 dimer 2 * 44000-45000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.179 beta-ketoacyl-acyl carrier protein synthases II
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 27
-
 assay at Escherichia coli
2.3.1.41 37
-
 assay at Escherichia coli