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Literature summary extracted from

  • Toomey, R.E.; Wakil, S.J.
    Studies on the mechanism of fatty acid synthesis. XVI. Preparation and general properties of acyl-malonyl acyl carrier protein-condensing enzyme from Escherichia coli (1966), J. Biol. Chem., 241, 1159-1165.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.41 2-mercaptoethanol activation Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.41 2-mercaptoethanol stabilizes Escherichia coli
2.3.1.41 EDTA stabilizes Escherichia coli
2.3.1.41 unstable during purification Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 acetyl-CoA
-
 Escherichia coli
2.3.1.41 acyl-CoA at high concentrations Escherichia coli
2.3.1.41 iodoacetamide acyl-[acyl-carrier-protein]-derivatives, e.g. acetyl-[acyl-carrier-protein], hexanoyl-CoA, octanoyl-CoA, decanoyl-CoA protect Escherichia coli
2.3.1.41 N-ethylmaleimide acyl-[acyl-carrier-protein]-derivatives protect Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 67600
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Escherichia coli part of non-associated fatty acid synthase system of plants and prokaryotes 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Escherichia coli involved in biosynthesis of saturated fatty acids 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 0.403
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.41 -20°C, complete loss of activity in the presence of EDTA and 2-mercaptoethanol Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] part of non-associated fatty acid synthase system of plants and prokaryotes Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] involved in biosynthesis of saturated fatty acids Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.41 7
-
 assay at Escherichia coli