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Literature summary extracted from

  • Nishida, I.; Frentzen, M.; Ishizaki, O.; Murata, N.
    Purification of isomeric forms of acyl-[acyl-carrier-protein]:glycerol-3-phosphate acyltransferase from greening squash cotyledons (1987), Plant Cell Physiol., 28, 1071-1079.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 chloroplast soluble Cucurbita moschata 9507
-
2.3.1.15 chloroplast stroma
-
Cucurbita moschata 9570
-
2.3.1.15 plasma membrane
-
Escherichia coli 5886
-
2.3.1.40 chloroplast stroma
-
Cucurbita moschata 9570
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 30000
-
isoenzyme AT1, gel filtration Cucurbita moschata
2.3.1.15 40000
-
isoenzymes AT2 and AT3, gel filtration Cucurbita moschata
2.3.1.40 30000
-
isozyme AT1, gel filtration Cucurbita moschata
2.3.1.40 40000
-
isozyme AT2 and isozyme AT3, gel filtration Cucurbita moschata
2.3.1.40 81000
-
-
Cucurbita moschata

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Cucurbita moschata
-
squash, isoenzymes AT1-3
-
2.3.1.15 Escherichia coli
-
-
-
2.3.1.40 Cucurbita moschata
-
squash
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 isoenzyme AT1, [acyl-carrier protein]-affinity chromatography, anion-exchange column, isoenzymes AT2 and AT3, [acyl-carrier protein]-affinity chromatography, hydroxylapatite Cucurbita moschata
2.3.1.40 acyl-carrier-protein affinity chromatography Cucurbita moschata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 cotyledon
-
Cucurbita moschata
-
2.3.1.40 cotyledon greening Cucurbita moschata
-

Subunits

EC Number Subunits Comment Organism
2.3.1.15 monomer 1 * 40000, isoenzymes AT2 and AT3, SDS-PAGE Cucurbita moschata
2.3.1.15 monomer 1 * 30000, isoenzyme AT1, SDS-PAGE Cucurbita moschata
2.3.1.40 monomer 1 * 30000, isozyme AT1, 1 * 40000, isozymes AT2 and AT3, SDS-PAGE Cucurbita moschata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.40 additional information
-
pI at 4°C: 5.5 (isozyme AT3), 5.6 (isozyme AT2), 6.6 (isozyme AT1) Cucurbita moschata