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Literature summary extracted from

  • Verwoert, II; Verhagen, E.F.; van der Linden, K.H.; Verbree, E.C.; Nijkamp, H.J.; Stuitje, A.R.
    Molecular characterization of an Escherichia coli mutant with a temperature-sensitive malonyl coenzyme A-acyl carrier protein transacylase (1994), FEBS Lett., 348, 311-316.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 cloning and nucleotide sequence analysis of the enzyme gene, overexpression of fabD89 gene in Escherichia coli Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.011
-
 malonyl-CoA wild-type and mutant Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 27000
-
 polypeptide encoded by the fabD89 allele which contains an amber mutation at codon position 257, enzymatically inactive Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Escherichia coli
-
-
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.39 42
-
 strain LA2-89 carrying the fabD89 allele which contains an amber mutation at codon position 257: the enzyme activity is almost completely inactivated by pre-incubation of the extract at this temperature Escherichia coli