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Literature summary extracted from

  • Rangan, V.S.; Serre, L.; Witkowska, H.E.; Bari, A.; Smith, S.
    Characterization of the malonyl-/acetyltransacylase domain of the multifunctional animal fatty acid synthase by expression in Escherichia coli and refolding in vitro (1997), Protein Eng., 10, 561-566.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expression in Escherichia coli of cDNAs of various lengths encoding the second domain of EC 2.3.1.85. Expression of a shortened transacylase, consisting of EC 2.3.1.85 residues 488-809 in Spodoptera frugiperda Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Rattus norvegicus
-
 malonyl transferase domain of EC 2.3.1.85
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 of cytosolic recombinant transacylase, resulting from the expression of DNA corresponding to residues 488-809 of EC 2.3.1.85 in Sf9 cells, purified with column chromatography on DE-53 anion exchanger, high-performance liquid chromatography on an Ultraspherogel SEC3000 and gel filtration Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.39 malonyl transferase domain of EC 2.3.1.85: refolding in vitro of the recombinant protein expressed in E. coli. A shortened transacylase, consisting of EC 2.3.1.85 residues 488-409 can be repeatedly denatured and renatured in vitro with reproducible high recovery and no loss in specific activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + pantetheine reaction catalysed by the malonyl transferase domain of EC 2.3.1.85 Rattus norvegicus malonyl-pantetheine + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.39 monomer malonyl transferase domain of EC 2.3.1.85, gel filtration Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 More in vertebrates, yeast and mycobacteria malonyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86 Rattus norvegicus