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Literature summary extracted from

  • Szafranska, A.E.; Hitchman, T.S.; Cox, R.J.; Crosby, J.; Simpson, T.J.
    Kinetic and mechanistic analysis of the malonyl CoA:ACP transacylase from Streptomyces coelicolor indicates a single catalytically competent serine nucleophile at the active site (2002), Biochemistry, 41, 1421-1427.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expressed in Escherichia coli as a hexahistidine-tagged fusion protein Streptomyces coelicolor

General Stability

EC Number General Stability Organism
2.3.1.39 enzyme labile during chromatography Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.39 phenylmethanesulfonyl fluoride
-
 Escherichia coli
2.3.1.39 phenylmethanesulfonyl fluoride
-
 Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.06
-
 malonyl-CoA hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein Streptomyces coelicolor
2.3.1.39 0.073
-
 acyl-carrier protein hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein Streptomyces coelicolor
2.3.1.39 0.25
-
 malonyl-CoA reaction with E. coli acyl-carrier-protein Escherichia coli
2.3.1.39 0.327
-
 malonyl-CoA hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein Streptomyces coelicolor
2.3.1.39 0.351
-
 acyl-carrier protein reaction with E. coli acyl-carrier-protein Escherichia coli
2.3.1.39 0.776
-
 acyl-carrier protein reaction with E. coli acyl-carrier-protein Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 34110
-
 electrospray mass spectrometry, hexahistidine-tagged fusion protein Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Escherichia coli
-
-
-
2.3.1.39 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 of the hexahistidine-tagged fusion protein, using Ni(II) affinity chromatography, column chromatography on Q-Sepharose, phenyl Superose and Mono-Q Streptomyces coelicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, kinetic studies Streptomyces coelicolor
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 additional information
-
-
 Escherichia coli
2.3.1.39 additional information
-
 hexahistidine-tagged fusion protein Streptomyces coelicolor
2.3.1.39 780
-
 hexahistidine-tagged fusion protein, at pH 6.5 and 30°C Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
 Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
 Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 30
-
 assay at Escherichia coli
2.3.1.39 30
-
 assay at Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.39 420
-
 malonyl CoA hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein Streptomyces coelicolor
2.3.1.39 450
-
 malonyl CoA hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein Streptomyces coelicolor
2.3.1.39 1580
-
 malonyl CoA reaction with E. coli acyl-carrier-protein Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.5
-
-
 Escherichia coli
2.3.1.39 6.5
-
 assay at Streptomyces coelicolor