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Literature summary extracted from

  • Ruch, F.E.; Vagelos, P.R.
    The isolation and general properties of Escherichia coli malonyl coenzyme A-acyl carrier protein transacylase (1973), J. Biol. Chem., 248, 8086-8094.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.39 dithiothreitol stabilizes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.39 iodoacetamide malonyl-CoA protects; pH-dependent Escherichia coli
2.3.1.39 additional information apparently homogeneous enzyme not inhibited by sulfhydryl agents, N-ethylmaleimide and iodoacetamide, highly purified enzyme stimulated by dithiothreitol Escherichia coli
2.3.1.39 N-ethylmaleimide malonyl-CoA protects; pH-dependent inhibition of purified enzyme Escherichia coli
2.3.1.39 phenylmethanesulfonyl fluoride
-
 Escherichia coli
2.3.1.39 sulfhydryl compounds purified enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 35000
-
 1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli
2.3.1.39 35500
-
 1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli
2.3.1.39 36500
-
 1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli
2.3.1.39 36660
-
 carboxymethylated enzyme: sedimentation equilibrium centrifugation Escherichia coli
2.3.1.39 37000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75, DEAE-Sephadex, and preparative polyacrylamide gel electrophoresis Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] isolation and characterization of an enzyme-intermediate Escherichia coli
a
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, formation of a malonyl-enzyme intermediate Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 1851
-
 purified enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
 Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.39 monomer 1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.5 8.5
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.39 5 10 about half-maximal activity at pH 5.0, about 80% of maximal activity at pH 10.0 Escherichia coli