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Literature summary extracted from

  • Lobo, S.; Florova, G.; Reynolds, K.A.
    A Streptomyces collinus thiolase with novel acetyl-CoA:acyl carrier protein transacylase activity (2001), Biochemistry, 40, 11955-11964.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9 expression in Escherichia coli Streptomyces collinus
2.3.1.38 cloning and sequencing of fadA gene, expression in Escherichia coli of wild-type and C92S mutant Streptomyces collinus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.9 C92S kcat decreases to 0.2% of that for the recombinant thiolase Streptomyces collinus
2.3.1.38 C92S no remaining activity acetyl-CoA:acyl carrier protein transacylase activity, but remaining strongly reduced thiolase activity Streptomyces collinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 thiolactomycin 0.36 mM, 50% inhibition Streptomyces collinus
2.3.1.38 iodoacetamide acetyl-CoA protects; strong Streptomyces collinus
2.3.1.38 thiolactomycin
-
 Streptomyces collinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.0038
-
 acetoacetyl-CoA native thiolase Streptomyces collinus
2.3.1.9 0.0062
-
 acetoacetyl-CoA C92S mutant thiolase Streptomyces collinus
2.3.1.9 0.0098
-
 acetoacetyl-CoA recombinant thiolase Streptomyces collinus
2.3.1.38 0.0043
-
 acetyl-CoA native wild-type enzyme Streptomyces collinus
2.3.1.38 0.0087
-
 acetyl-CoA recombinant wild-type enzyme Streptomyces collinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 185000
-
 native thiolase, gel filtration Streptomyces collinus
2.3.1.9 187000
-
 recombinant thiolase, gel filtration Streptomyces collinus
2.3.1.38 additional information
-
 amino acid sequence, comparison Streptomyces collinus
2.3.1.38 45000
-
 4 * 45000, SDS-PAGE Streptomyces collinus
2.3.1.38 185000
-
 gel filtration Streptomyces collinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Streptomyces collinus
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Streptomyces collinus
-
-
-
2.3.1.38 Streptomyces collinus
-
 gene fadA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 Q-Sepharose, ammonium sulfate, Sephacryl S-200, Mono Q, FPLC S-300 Streptomyces collinus
2.3.1.38 native wild-type and recombinant wild-type and mutant from E. coli Streptomyces collinus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 36.6
-
-
 Streptomyces collinus
2.3.1.38 0.022
-
 purified native wild-type enzyme Streptomyces collinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Streptomyces collinus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA enzyme has both thiolase and acetyl-CoA:acyl carrier protein transacylase activity Streptomyces collinus acetyl-CoA + acetyl-CoA
-
 r
2.3.1.38 acetyl-CoA + [acyl-carrier protein] specific for acetyl-CoA, utilizes [acyl-carrier-protein] of diverse origins with differing affinities Streptomyces collinus CoA + acetyl-[acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 46000-48000, recombinant thiolase, SDS-PAGE Streptomyces collinus
2.3.1.9 tetramer 4 * 45000-47000, native thiolase, SDS-PAGE Streptomyces collinus
2.3.1.38 tetramer 4 * 45000, SDS-PAGE Streptomyces collinus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.38 acetyl-CoA:acyl carrier protein transacylase
-
 Streptomyces collinus
2.3.1.38 ACT
-
 Streptomyces collinus
2.3.1.38 More cf. EC 2.3.1.85 and EC 2.3.1.86 Streptomyces collinus
2.3.1.38 More enzyme has both a acetyl-CoA:acyl carrier protein transacylase activity and a much higher thiolase activity Streptomyces collinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.38 30
-
 assay at Streptomyces collinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.9 60
-
 15 min, loss of 47% activity Streptomyces collinus
2.3.1.38 additional information
-
-
 Streptomyces collinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.9 0.0767
-
 acetoacetyl-CoA C92S mutant thiolase Streptomyces collinus
2.3.1.9 36.2
-
 acetoacetyl-CoA native thiolase Streptomyces collinus
2.3.1.9 40.1
-
 acetoacetyl-CoA recombinant thiolase Streptomyces collinus
2.3.1.38 0.0225
-
 acetyl-CoA recombinant wild-type, crude Streptomyces glaucescens [acyl-carrier-protein] Streptomyces collinus
2.3.1.38 0.0508
-
 acetyl-CoA native wild-type, crude E. coli [acyl-carrier-protein] Streptomyces collinus