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Literature summary extracted from

  • Lowe, P.N.; Rhodes, S.
    Purification and characterization of [acyl-carrier-protein] acetyltransferase from Escherichia coli (1988), Biochem. J., 250, 789-796.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.38 acetyl-CoA at high concentration Escherichia coli
2.3.1.38 acyl-carrier protein at high concentration Escherichia coli
2.3.1.38 CoA competitive Escherichia coli
2.3.1.38 iodoacetamide acetyl-CoA protects Escherichia coli
2.3.1.38 methyl methanethiosulfonate weak Escherichia coli
2.3.1.38 additional information no inhibition by PMSF Escherichia coli
2.3.1.38 N-ethylmaleimide acetyl-CoA protects Escherichia coli
2.3.1.38 pantetheine weak, competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.38 0.035
-
 acyl-carrier protein
-
 Escherichia coli
2.3.1.38 0.05
-
 acetyl-CoA
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.38 29000
-
 2 * 29000, SDS-PAGE Escherichia coli
2.3.1.38 61000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.38 Escherichia coli
-
 B
-
2.3.1.38 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.38
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.38 acetyl-CoA + an [acyl-carrier protein] = CoA + an acetyl-[acyl-carrier protein] Bi Bi ping pong mechanism, acetyl-enzyme intermediate Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.38 0.8
-
 approx., purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.38 -20°C, several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.38 acetyl-CoA + [acyl-carrier protein] via acetyl-enzyme intermediate Escherichia coli CoA + acetyl-[acyl-carrier protein]
-
 r
2.3.1.38 acetyl-CoA + [acyl-carrier protein] via acetyl-enzyme intermediate Escherichia coli B / ATCC 11303 CoA + acetyl-[acyl-carrier protein]
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.38 dimer 2 * 29000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.38 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.38 6.5
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.38 0.03
-
 CoA
-
 Escherichia coli
2.3.1.38 3.2
-
 pantetheine
-
 Escherichia coli