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Literature summary extracted from

  • Jordan, P.M.; Laghai-Newton, A.
    Purification of 5-aminolevulinate synthase (1986), Methods Enzymol., 123, 435-443.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.37 aminomalonate
-
 Cereibacter sphaeroides
2.3.1.37 Cu2+
-
 Cereibacter sphaeroides
2.3.1.37 Fe2+
-
 Cereibacter sphaeroides
2.3.1.37 hemin reversible, noncompetitive Cereibacter sphaeroides
2.3.1.37 p-chloromercuribenzoate
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.37 49000
-
 2 * 49000, SDS-PAGE Cereibacter sphaeroides
2.3.1.37 81000
-
 gel filtration Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37
-
 Cereibacter sphaeroides

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.37 succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 mechanism Cereibacter sphaeroides
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.37 2.22
-
 purified enzyme Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 acetyl-CoA + glycine low activity Cereibacter sphaeroides ?
-
 ?
2.3.1.37 butyryl-CoA + glycine low activity Cereibacter sphaeroides ?
-
 ?
2.3.1.37 propionyl-CoA + glycine low activity Cereibacter sphaeroides ?
-
 ?
2.3.1.37 succinyl-CoA + glycine absolute requirement for glycine Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine enzyme utilizes acetyl-CoA, propionyl-CoA and butyryl-CoA at much lower rates Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.37 dimer 2 * 49000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.37 37
-
 assay at Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate fully active with 0.05 mM pyridoxal 5'-phosphate Cereibacter sphaeroides
2.3.1.37 pyridoxal 5'-phosphate weekly bound Cereibacter sphaeroides