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Literature summary extracted from

  • Liu, Y.; Van Heeswijck, R.; Hoj, P.; Hoogenraad, N.
    Purification and characterization of ornithine acetyltransferase from Saccharomyces cerevisiae (1995), Eur. J. Biochem., 228, 291-296.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.35 1
-
 alpha-N-acetyl-L-ornithine
-
 Saccharomyces cerevisiae
2.3.1.35 1.5
-
 L-ornithine
-
 Saccharomyces cerevisiae
2.3.1.35 7.2
-
 L-glutamate
-
 Saccharomyces cerevisiae
2.3.1.35 17.1
-
 N-acetylglutamate
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.35 mitochondrial matrix
-
 Saccharomyces cerevisiae 5759
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.35 26000
-
 alphabeta, 1 * 31000 + 1 * 26000, SDS-PAGE Saccharomyces cerevisiae
2.3.1.35 31000
-
 alphabeta, 1 * 31000 + 1 * 26000, SDS-PAGE Saccharomyces cerevisiae
2.3.1.35 57000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Saccharomyces cerevisiae first step in arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.35 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.35
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.35 22
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.3.1.35 -20°C, 10% loss activity per week Saccharomyces cerevisiae
2.3.1.35 4°C, 10% loss activity per week Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Saccharomyces cerevisiae L-ornithine + N-acetyl-L-glutamate
-
 r
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate first step in arginine biosynthesis Saccharomyces cerevisiae L-ornithine + N-acetyl-L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.35 heterodimer alphabeta, 1 * 31000 + 1 * 26000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.35 7.5
-
-
 Saccharomyces cerevisiae