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Literature summary extracted from

  • Born, T.L.; Franklin, M.; Blanchard, J.S.
    Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Haemophilus influenzae met2-encoded homoserine transacetylase (2000), Biochemistry, 39, 8556-8564.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.31 met2 gene, expression in Escherichia coli BL21 (DE3) Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.31 0.09
-
 propionyl-CoA
-
 Haemophilus influenzae
2.3.1.31 0.13
-
 L-homoserine
-
 Haemophilus influenzae
2.3.1.31 0.13
-
 crotonyl-CoA
-
 Haemophilus influenzae
2.3.1.31 0.14
-
 acetyl-CoA
-
 Haemophilus influenzae
2.3.1.31 0.21
-
 butyryl-CoA
-
 Haemophilus influenzae
2.3.1.31 0.28
-
 glutaryl-CoA
-
 Haemophilus influenzae
2.3.1.31 0.36
-
 succinyl-CoA
-
 Haemophilus influenzae
2.3.1.31 0.62
-
 CoA
-
 Haemophilus influenzae
2.3.1.31 0.85
-
 O-acetyl-L-homoserine
-
 Haemophilus influenzae
2.3.1.31 1.4
-
 4-nitrophenyl acetate
-
 Haemophilus influenzae
2.3.1.31 4.7
-
 D-homoserine
-
 Haemophilus influenzae
2.3.1.31 19
-
 4-hydroxybutyric acid
-
 Haemophilus influenzae
2.3.1.31 71
-
 3-Amino-1-propanol
-
 Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.31 73000
-
 gel filtration Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine Haemophilus influenzae control point in the pathway CoA + O-acetyl-L-homoserine
-
 r
2.3.1.31 acetyl-CoA + L-homoserine Haemophilus influenzae first reaction in L-methionine biosynthesis CoA + O-acetyl-L-homoserine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.31 Haemophilus influenzae
-
 MET2 gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.31 recombinant from E. coli Haemophilus influenzae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.31 acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine ping-pong reaction mechanism Haemophilus influenzae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.31 acetyl-CoA + 3-amino-1-propanol low activity Haemophilus influenzae ?
-
 r
2.3.1.31 acetyl-CoA + 4-nitrophenyl acetate low activity Haemophilus influenzae ?
-
 r
2.3.1.31 acetyl-CoA + D-homoserine
-
 Haemophilus influenzae CoA + O-acetyl-D-homoserine
-
 r
2.3.1.31 acetyl-CoA + gamma-hydroxybutyric acid
-
 Haemophilus influenzae ?
-
 r
2.3.1.31 acetyl-CoA + L-homoserine preference for acetyl-CoA Haemophilus influenzae CoA + O-acetyl-L-homoserine
-
 r
2.3.1.31 acetyl-CoA + L-homoserine control point in the pathway Haemophilus influenzae CoA + O-acetyl-L-homoserine
-
 r
2.3.1.31 acetyl-CoA + L-homoserine first reaction in L-methionine biosynthesis Haemophilus influenzae CoA + O-acetyl-L-homoserine
-
 r
2.3.1.31 butyryl-CoA + L-homoserine
-
 Haemophilus influenzae CoA + O-butyryl-L-homoserine
-
 ?
2.3.1.31 crotonyl-CoA + L-homoserine low activity Haemophilus influenzae CoA + O-crotonyl-L-homoserine
-
 r
2.3.1.31 glutaryl-CoA + L-homoserine very low activity Haemophilus influenzae CoA + O-glutaryl-L-homoserine
-
 r
2.3.1.31 propionyl-CoA + L-homoserine
-
 Haemophilus influenzae CoA + O-propionyl-L-homoserine
-
 ?
2.3.1.31 succinyl-CoA + L-homoserine very low activity Haemophilus influenzae CoA + O-succinyl-L-homoserine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.31 dimer 2 * 35000-40000, SDS-PAGE and electrospray ionization-mass spectrometry Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.31 25
-
 assay at Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.31 5
-
 3-Amino-1-propanol
-
 Haemophilus influenzae
2.3.1.31 7.8
-
 4-nitrophenyl acetate
-
 Haemophilus influenzae
2.3.1.31 15
-
 crotonyl-CoA
-
 Haemophilus influenzae
2.3.1.31 27
-
 butyryl-CoA
-
 Haemophilus influenzae
2.3.1.31 30
-
 propionyl-CoA
-
 Haemophilus influenzae
2.3.1.31 31
-
 O-acetyl-L-homoserine
-
 Haemophilus influenzae
2.3.1.31 50
-
 4-hydroxybutyric acid
-
 Haemophilus influenzae
2.3.1.31 78
-
 D-homoserine
-
 Haemophilus influenzae
2.3.1.31 92
-
 acetyl-CoA
-
 Haemophilus influenzae
2.3.1.31 92
-
 L-homoserine
-
 Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.31 7.5
-
 assay at Haemophilus influenzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.31 5.5 9
-
 Haemophilus influenzae