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Literature summary extracted from

  • Noji, M.; Saito, K.
    Molecular and biochemical analysis of serine acetyltransferase and cysteine synthase towards sulfur metabolic engineering in plants (2002), Amino Acids, 22, 231-243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 expression of wild-type and mutants in Escherichia coli Citrullus lanatus subsp. vulgaris
2.5.1.47 construction of transgenic Nicotiana tabacum carrying either spinach cytosolic cDNA, designated 3F plants, or chimeric CSAse A cDNA fused with the sequence for chloroplast-targeting transit peptide of pea Rubisco small subunit, designated 4F plants. Generation of F1 transgenic tobacco, highly tolerant to sulfur-containing pollutants, in which Csase activities are enhanced both in cytosol and in the chloroplasts by crossing 3F plants with 4F plants Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.30 E278L site-directed mutagenesis, about 1.7fold more sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
2.3.1.30 F285Y site-directed mutagenesis, about 1.7fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
2.3.1.30 G277C site-directed mutagenesis, about 27fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
2.3.1.30 H282Q site-directed mutagenesis, about 3.5fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
2.3.1.30 additional information SAT of transgenic Arabidopsis plants overexpressing the point-mutated watermelon gene are not inhibited by L-cysteine, regardless if the recombinant enzyme is targeted to the chloroplast or expressed in the cytosol Arabidopsis thaliana
2.3.1.30 additional information SAT of transgenic Arabidopsis plants overexpressing the point-mutated watermelon gene are not inhibited by L-cysteine, regardless if the recombinant enzyme is targeted to the chloroplast or expressed in the cytosol Citrullus lanatus subsp. vulgaris
2.3.1.30 additional information transgenic Arabidopsis plants expressing recombinant wild-type SATase are inhibited by L-cysteine Arabidopsis thaliana
2.3.1.30 additional information transgenic Arabidopsis plants expressing recombinant wild-type SATase are inhibited by L-cysteine Citrullus lanatus subsp. vulgaris
2.3.1.30 S279T site-directed mutagenesis, as sensitive to inhibition by L-cysteine as the wild-type Citrullus lanatus subsp. vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.30 L-cysteine feed-back inhibition; weak Allium tuberosum
2.3.1.30 L-cysteine feed-back inhibition of cytosolic isozyme SAT-c, not of mitochondrial and plastidic isozymes SAT-m and SAT-p Arabidopsis thaliana
2.3.1.30 L-cysteine feed-back inhibition Citrullus lanatus subsp. vulgaris
2.3.1.30 L-cysteine feed-back inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.30 additional information
-
 additional information
-
 Arabidopsis thaliana
2.3.1.30 additional information
-
 additional information Km for L-serine and acetyl-CoA of recombinant L-cysteine-binding motif mutants, overview Citrullus lanatus subsp. vulgaris
2.3.1.30 0.13
-
 acetyl-CoA
-
 Arabidopsis thaliana
2.3.1.30 0.13
-
 acetyl-CoA
-
 Citrullus lanatus subsp. vulgaris
2.3.1.30 0.59
-
 L-serine
-
 Arabidopsis thaliana
2.3.1.30 0.59
-
 L-serine
-
 Citrullus lanatus subsp. vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.30 chloroplast
-
 Spinacia oleracea 9507
-
2.3.1.30 chloroplast
-
 Arabidopsis thaliana 9507
-
2.3.1.30 chloroplast
-
 Citrullus lanatus subsp. vulgaris 9507
-
2.3.1.30 chloroplast
-
 Allium tuberosum 9507
-
2.3.1.30 cytosol
-
 Spinacia oleracea 5829
-
2.3.1.30 cytosol
-
 Citrullus lanatus subsp. vulgaris 5829
-
2.3.1.30 cytosol
-
 Allium tuberosum 5829
-
2.3.1.30 cytosol isozyme SAT-c Arabidopsis thaliana 5829
-
2.3.1.30 mitochondrion
-
 Citrullus lanatus subsp. vulgaris 5739
-
2.3.1.30 mitochondrion
-
 Allium tuberosum 5739
-
2.3.1.30 mitochondrion isozyme SAT-m, matrix Arabidopsis thaliana 5739
-
2.5.1.47 cytosol
-
 Spinacia oleracea 5829
-
2.5.1.47 cytosol
-
 Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Spinacia oleracea key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine Citrullus lanatus subsp. vulgaris key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine Allium tuberosum key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir
2.5.1.47 O-acetyl-L-Ser + sulfide Arabidopsis thaliana
-
 L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide Spinacia oleracea final step in Cys synthesis L-Cys + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Allium tuberosum
-
 Chinese chive
-
2.3.1.30 Arabidopsis thaliana
-
 3 isozymes specific for subcellular compartment: SAT-c, SAT-m, SAT-p
-
2.3.1.30 Citrullus lanatus subsp. vulgaris
-
-
-
2.3.1.30 Spinacia oleracea
-
-
-
2.5.1.47 Arabidopsis thaliana
-
-
-
2.5.1.47 Spinacia oleracea
-
-
-
2.5.1.47 Triticum aestivum
-
-
-
2.5.1.47 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Spinacia oleracea CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine
-
 Citrullus lanatus subsp. vulgaris CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine
-
 Allium tuberosum CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Spinacia oleracea CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Citrullus lanatus subsp. vulgaris CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Allium tuberosum CoA + O-acetyl-L-serine
-
 ir
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Spinacia oleracea L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Arabidopsis thaliana L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide
-
 Arabidopsis thaliana L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Spinacia oleracea L-Cys + acetate
-
 ?