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Literature summary extracted from

  • Kredich, N.M.; Tomkins, G.M.
    The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium (1966), J. Biol. Chem., 241, 4955-4965.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.30 additional information
-
 Escherichia coli
2.3.1.30 additional information CysE mutants show strongly reduced enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.30 L-cysteic acid
-
 Escherichia coli
2.3.1.30 L-cysteine
-
 Escherichia coli
2.3.1.30 L-cysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.30 L-serine above 3 mM, weak Escherichia coli
2.3.1.30 L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.30 N-acetyl-L-cysteine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.30 0.11
-
 L-serine
-
 Escherichia coli
2.3.1.30 0.56
-
 acetyl-CoA
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.30 additional information salt concentrations less than 0.02 M or greater than 0.2 M appreciably decrease reaction rate Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Salmonella enterica subsp. enterica serovar Typhimurium first enzyme in L-cysteine synthesis pathway CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine Escherichia coli first enzyme in L-cysteine synthesis pathway CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine Escherichia coli B / ATCC 11303 first enzyme in L-cysteine synthesis pathway CoA + O-acetyl-L-serine
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Escherichia coli
-
 B
-
2.3.1.30 Escherichia coli B / ATCC 11303
-
 B
-
2.3.1.30 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine first enzyme in L-cysteine synthesis pathway Salmonella enterica subsp. enterica serovar Typhimurium CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine first enzyme in L-cysteine synthesis pathway Escherichia coli CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine
-
 Escherichia coli B / ATCC 11303 CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine first enzyme in L-cysteine synthesis pathway Escherichia coli B / ATCC 11303 CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-threonine 0.5% of the activity with L-serine Escherichia coli CoA + O-acetyl-L-threonine
-
 ?
2.3.1.30 acetyl-CoA + L-threonine 0.5% of the activity with L-serine Escherichia coli B / ATCC 11303 CoA + O-acetyl-L-threonine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.30 7.6 7.8
-
 Escherichia coli