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Literature summary extracted from

  • Shimeno, H.; Soeda, S.; Sakamoto, M.; Kouchi, T.; Kowakame, T.; Kihara, T.
    Partial purification and characterization of sphingosine N-acyltransferase (ceramide synthase) from bovine liver mitochondrion-rich fraction (1998), Lipids, 33, 601-605.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.24 0.141
-
 palmitoyl-CoA
-
 Bos taurus
2.3.1.24 0.144
-
 sphinganine
-
 Bos taurus
2.3.1.24 0.146
-
 stearoyl-CoA
-
 Bos taurus
2.3.1.24 0.171
-
 sphingosine
-
 Bos taurus
2.3.1.24 0.18
-
 oleoyl-CoA
-
 Bos taurus
2.3.1.24 0.299
-
 behenoyl-CoA
-
 Bos taurus
2.3.1.24 0.358
-
 lauroyl-CoA
-
 Bos taurus
2.3.1.24 0.522
-
 hexanoyl-CoA
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.24 62000
-
 x * 62000 + x * 72000, SDS-PAGE Bos taurus
2.3.1.24 72000
-
 x * 62000 + x * 72000, SDS-PAGE Bos taurus
2.3.1.24 240000 260000 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.24 n-octyl beta-D-thioglucoside, DE-32, sphingosine-celite, Sepharose CL-6B Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 brain
-
 Bos taurus
-
2.3.1.24 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.24 0.0148
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 an acyl-CoA + sphinganine
-
 Bos taurus CoA + an N-acylsphinganine
-
 ?
2.3.1.24 an acyl-CoA + sphingosine
-
 Bos taurus CoA + an N-acylsphingosine
-
 ?
2.3.1.24 behenoyl-CoA + sphingosine
-
 Bos taurus CoA + N-behenoylsphingosine
-
 ?
2.3.1.24 hexanoyl-CoA + sphingosine
-
 Bos taurus CoA + N-hexanoylsphingosine
-
 ?
2.3.1.24 lauroyl-CoA + sphingosine
-
 Bos taurus CoA + N-lauroylsphingosine
-
 ?
2.3.1.24 oleoyl-CoA + sphingosine
-
 Bos taurus CoA + N-oleoylsphingosine
-
 ?
2.3.1.24 palmitoyl-CoA + sphingosine
-
 Bos taurus CoA + N-palmitoylsphingosine
-
 ?
2.3.1.24 stearoyl-CoA + sphingosine
-
 Bos taurus CoA + N-stearoylsphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.24 ? x * 62000 + x * 72000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.24 5.5 9 more than 70% of maximal activity between pH 7.0 and pH 8.0 Bos taurus