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Literature summary extracted from

  • Merrill, A.H.; Wang, E.
    Enzymes of ceramide biosynthesis (1992), Methods Enzymol., 209, 427-437.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.50
-
 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.50 beta-Haloalanines
-
 Rattus norvegicus
2.3.1.50 L-Cycloserine
-
 Rattus norvegicus
2.3.1.50 L-Cycloserine
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.50 0.1 1 L-serine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.50 microsome
-
 Rattus norvegicus
-
-
2.3.1.50 microsome
-
 Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Rattus norvegicus initial step of de novo ceramide biosynthesis CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine Saccharomyces cerevisiae initial step of de novo ceramide biosynthesis CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine Prevotella melaninogenica initial step of de novo ceramide biosynthesis CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Prevotella melaninogenica
-
-
-
2.3.1.50 Rattus norvegicus
-
-
-
2.3.1.50 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.50 additional information
-
-
 Saccharomyces cerevisiae
2.3.1.50 additional information
-
 assay method Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine
-
 Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine
-
 Prevotella melaninogenica CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Rattus norvegicus CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine no other amino acids can substitute for serine Rattus norvegicus CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine initial step of de novo ceramide biosynthesis Rattus norvegicus CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine initial step of de novo ceramide biosynthesis Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine initial step of de novo ceramide biosynthesis Prevotella melaninogenica CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.50 37
-
 assay at Rattus norvegicus
2.3.1.50 37
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate required for activity Saccharomyces cerevisiae
2.3.1.50 pyridoxal 5'-phosphate pyridoxal 5'-phosphate can be resolved from the enzyme by dialysis Rattus norvegicus