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Literature summary extracted from

  • Choy, P.C.; Skrzypczak, M.; Lee, D.; Jay, F.T.
    Acyl-GPC and alkenyl/alkyl-GPC:acyl-CoA acyltransferases (1997), Biochim. Biophys. Acta, 1348, 124-133.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.25 Ca2+ enhances activity of microsomal enzyme Cavia porcellus
2.3.1.25 Mg2+ enhances activity of microsomal enzyme Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.25 1-acyl-glycero-3-phosphorylcholine non competitive Cavia porcellus
2.3.1.25 Ca2+ mitochondrial enzyme Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.25 microsome
-
 Cavia porcellus
-
-
2.3.1.25 mitochondrion
-
 Cavia porcellus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.23 43000
-
 x * 43000, brain enzyme, SDS-PAGE Bos taurus
2.3.1.23 64000
-
 x * 64000, heart enzyme, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.25 linoleoyl-CoA + 1-alkenyl-glycero-3-phosphorylcholine Cavia porcellus
-
 CoA + 1-alkenyl-2-linoleoyl-glycero-3-phosphorylcholine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Bos taurus
-
-
-
2.3.1.25 Cavia porcellus
-
 guinea pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.23 Spharose 6B, Mono Q, severe loss of activity is partially avoided by incorporation of 20% glycerol in the elution buffer Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.25 heart
-
 Cavia porcellus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.23 -70°C, several weeks, no loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
 Bos taurus CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.25 linoleoyl-CoA + 1-alkenyl-glycero-3-phosphorylcholine
-
 Cavia porcellus CoA + 1-alkenyl-2-linoleoyl-glycero-3-phosphorylcholine
-
 r
2.3.1.25 linoleoyl-CoA + 1-alkenyl-glycero-3-phosphorylcholine heart enzyme is highly specific for linoleoyl-CoA Cavia porcellus CoA + 1-alkenyl-2-linoleoyl-glycero-3-phosphorylcholine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.23 ? x * 43000, brain enzyme, SDS-PAGE Bos taurus
2.3.1.23 ? x * 64000, heart enzyme, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.25 55
-
 90% loss of activity after 1 min Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.25 6 7
-
 Cavia porcellus