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Literature summary extracted from

  • Coleman, R.A.; Haynes, E.B.
    Monoacylglycerol acyltransferase. Evidence that the activities from rat intestine and suckling liver are tissue-specific isoenzymes (1986), J. Biol. Chem., 261, 224-228.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.22 bovine serum albumin activation, liver, below 1 mg/ml, inhibitory above, not intestine Rattus norvegicus
2.3.1.22 deoxycholate stimulates enzyme from intestinal mucosa Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.22 2,4,6-Trinitrobenzene sulfonic acid 3 mM, 80% inactivation of liver enzyme, 45% inactivation of intestinal enzyme, palmitate partly protects, enzyme from intestine is less sensitive than enzyme from liver Rattus norvegicus
2.3.1.22 2-monooleoylglycerol high concentration Rattus norvegicus
2.3.1.22 acetone 10%, liver Rattus norvegicus
2.3.1.22 bovine serum albumin activation below 1 mg/ml Rattus norvegicus
2.3.1.22 deoxycholate inhibition of liver enzyme Rattus norvegicus
2.3.1.22 diethyldicarbonate 0.04 mM, 50% inhibition, 0.05 mM palmitate partly protects, completely reversible by 50 mM hydroxylamine, enzyme from intestine is less sensitive than enzyme from liver Rattus norvegicus
2.3.1.22 EDTA weak Rattus norvegicus
2.3.1.22 HgCl2 weak Rattus norvegicus
2.3.1.22 MnCl2 2.5 mM, 86% inhibition of the intestinal enzyme, 40% inhibition of the enzyme from suckling liver Rattus norvegicus
2.3.1.22 palmitoyl-CoA high concentration Rattus norvegicus
2.3.1.22 PCMB
-
 Rattus norvegicus
2.3.1.22 taurocholate
-
 Rattus norvegicus
2.3.1.22 Triton X-100
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.22 microsome
-
 Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.22 acyl-CoA + sn-2-monoacylglycerol Rattus norvegicus involved in synthesis of triacylglycerols CoA + diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.22 Rattus norvegicus
-
 Zivic Miller SD-strain
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.22 liver neonatal Rattus norvegicus
-
2.3.1.22 additional information tissue specific isozymes Rattus norvegicus
-
2.3.1.22 small intestine mucosa
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.22 acyl-CoA + sn-2-monoacylglycerol involved in synthesis of triacylglycerols Rattus norvegicus CoA + diacylglycerol
-
 ?
2.3.1.22 fatty acyl-CoA + sn-2-monoacylglycerol
-
 Rattus norvegicus CoA + 1,2-diacylglycerol
-
 ?
2.3.1.22 palmitoyl-CoA + rac-1-monooleoylglycerol intestine, at 78% the rate of sn-2-monooleoylglycerol acylation Rattus norvegicus CoA + ?
-
 ?
2.3.1.22 palmitoyl-CoA + rac-1-monooleoylglycerol ether intestine, at 45% the rate of sn-2-monooleoylglycerol acylation Rattus norvegicus CoA + ?
-
 ?
2.3.1.22 palmitoyl-CoA + sn-2-monooleoylglycerol intestine, at 52% the rate of sn-2-monooleoylglycerol acylation Rattus norvegicus CoA + 1(3)-palmitoyl-2-oleoylglycerol
-
 ?
2.3.1.22 palmitoyl-CoA + sn-2-monooleoylglycerol liver enzyme highly specific for sn-2-monooleoylglycerol Rattus norvegicus CoA + 1(3)-palmitoyl-2-oleoylglycerol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.22 48
-
 12 min, microsomes from liver retain 90% of the initial activity Rattus norvegicus
2.3.1.22 51
-
 12 min, microsomes from liver lose 23% of the initial activity, microsomes from intestinal mucosa lose 88.5% of control activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.22 7 9
-
 Rattus norvegicus