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Literature summary extracted from

  • Chohnan, S.; Fujio, T.; Takaki, T.; Yonekura, M.; Nishihara, H.; Takamura, Y.
    Malonate decarboxylase of Pseudomonas putida is composed of five subunits (1998), FEMS Microbiol. Lett., 169, 37-43.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 13000
-
 malonate decarboxylase holoenzyme is a heteropentamer, the active form: 1 * 60000, alpha-subunit, plus 1 * 33000, beta-subunit, plus 1 * 28000, gamma subunit, plus 1 * 13000, delta-subunit, plus 1 * 30000, epsilon-subunit. The epsilon subunit functions as malonyl-CoA:ACP transacylase Pseudomonas putida
2.3.1.39 28000
-
 malonate decarboxylase holoenzyme is a heteropentamer, the active form: 1 * 60000, alpha-subunit, plus 1 * 33000, beta-subunit, plus 1 * 28000, gamma subunit, plus 1 * 13000, delta-subunit, plus 1 * 30000, epsilon-subunit. The epsilon subunit functions as malonyl-CoA:ACP transacylase Pseudomonas putida
2.3.1.39 30000
-
 malonate decarboxylase holoenzyme is a heteropentamer, the active form: 1 * 60000, alpha-subunit, plus 1 * 33000, beta-subunit, plus 1 * 28000, gamma subunit, plus 1 * 13000, delta-subunit, plus 1 * 30000, epsilon-subunit. The epsilon subunit functions as malonyl-CoA:ACP transacylase Pseudomonas putida
2.3.1.39 33000
-
 malonate decarboxylase holoenzyme is a heteropentamer, the active form: 1 * 60000, alpha-subunit, plus 1 * 33000, beta-subunit, plus 1 * 28000, gamma subunit, plus 1 * 13000, delta-subunit, plus 1 * 30000, epsilon-subunit. The epsilon subunit functions as malonyl-CoA:ACP transacylase Pseudomonas putida
2.3.1.39 60000
-
 malonate decarboxylase holoenzyme is a heteropentamer, the active form: 1 * 60000, alpha-subunit, plus 1 * 33000, beta-subunit, plus 1 * 28000, gamma subunit, plus 1 * 13000, delta-subunit, plus 1 * 30000, epsilon-subunit. The epsilon subunit functions as malonyl-CoA:ACP transacylase Pseudomonas putida
2.3.1.39 130000
-
 gel filtration, inactive form of malonate decarboxylase Pseudomonas putida
2.3.1.39 165000
-
 gel filtration, active form of malonate decarboxylase Pseudomonas putida
4.1.1.9 13000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, inactive enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 13000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, + 1 * epsilon, 30000, active enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 28000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, inactive enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 28000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, + 1 * epsilon, 30000, active enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 33000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, inactive enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 33000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, + 1 * epsilon, 30000, active enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 60000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, inactive enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 60000
-
 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, + 1 * epsilon, 30000, active enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 130000
-
 inactive enzyme form, gel filtration Pseudomonas putida
4.1.1.9 165000
-
 active enzyme form, gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Pseudomonas putida Q9Z447
-
-
4.1.1.9 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39
-
 Pseudomonas putida
4.1.1.9
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 acetoacetyl-CoA + acyl-carrier protein
-
 Pseudomonas putida CoA + acetoacetyl-[acyl-carrier protein]
-
 ?
2.3.1.39 butanoyl-CoA + acyl-carrier protein
-
 Pseudomonas putida CoA + butanoyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
 Pseudomonas putida CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 methylmalonyl-CoA + acyl-carrier protein
-
 Pseudomonas putida CoA + methylmalonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 propionyl-CoA + acyl-carrier protein
-
 Pseudomonas putida CoA + propionyl-[acyl-carrier protein]
-
 ?
4.1.1.9 Malonyl-CoA
-
 Pseudomonas putida Acetyl-CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.39 pentamer malonate decarboxylase holoenzyme is a heteropentamer, the active form: 1 * 60000, alpha-subunit, plus 1 * 33000, beta-subunit, plus 1 * 28000, gamma subunit, plus 1 * 13000, delta-subunit, plus 1 * 30000, epsilon-subunit. The epsilon subunit functions as malonyl-CoA:ACP transacylase Pseudomonas putida
4.1.1.9 pentamer 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, + 1 * epsilon, 30000, active enzyme form, SDS-PAGE Pseudomonas putida
4.1.1.9 tetramer 1 * 60000, alpha, + 1 * 33000, beta, + 1 * 28000, gamma, + 1 * 13000, delta, inactive enzyme form, SDS-PAGE Pseudomonas putida