Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nicot, C.; Hegardt, F.G.; Woldegiorgis, G.; Haro, D.; Marrero, P.F.
    Pig liver carnitine palmitoyltransferase I, with low Km for carnitine and high sensitivity to malonyl-CoA inhibition, is a natural chimera of rat liver and muscle enzymes (2001), Biochemistry, 40, 2260-2266.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 2 isoforms of CPT I, liver L-CPT I, skeletal muscle M-CPT I, cloned from liver, expression in Pichia pastoris GS115, DNA sequence determination Sus scrofa
2.3.1.21 in vitro transcription and translation of L-CPT I Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA L-CPT I Sus scrofa
2.3.1.21 additional information L-CPT I behaves like a natural chimera of M-CPT I and L-CPT I due to malonyl-CoA sensitivity and Km values compared to the enzymes of other organisms Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 additional information
-
 additional information L-CPT I behaves like a natural chimera of M-CPT I and L-CPT I due to malonyl-CoA sensitivity and Km values compared to the enzymes of other organisms Sus scrofa
2.3.1.21 0.035
-
 palmitoyl-CoA recombinant wild-type L-CPT I Sus scrofa
2.3.1.21 0.126
-
 L-carnitine recombinant wild-type L-CPT I Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.21 mitochondrial outer membrane CPT I is located in the outer membrane Sus scrofa 5741
-
2.3.1.21 mitochondrion 2 isoforms of CPT I, L-CPT I and M-CPT I Sus scrofa 5739
-
2.3.1.21 mitochondrion recombinant L-CPT I and M-CPT I in Pichia pastoris Sus scrofa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.21 82000
-
 x * 82000, L-CPT I, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine Sus scrofa
-
 CoA + L-acylcarnitine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Sus scrofa
-
 2 isoforms of CPT I in liver mitochondria, liver L-CPT I, skeletal muscle M-CPT I
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.21 duodenum L-CPT I Sus scrofa
-
2.3.1.21 heart L-CPT I Sus scrofa
-
2.3.1.21 heart M-CPT I Sus scrofa
-
2.3.1.21 kidney L-CPT I Sus scrofa
-
2.3.1.21 liver L-CPT I Sus scrofa
-
2.3.1.21 liver M-CPT I Sus scrofa
-
2.3.1.21 liver CPT I Sus scrofa
-
2.3.1.21 skeletal muscle M-CPT I Sus scrofa
-
2.3.1.21 testis L-CPT I, not M-CPT I Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine
-
 Sus scrofa CoA + L-acylcarnitine
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine
-
 Sus scrofa CoA + L-palmitoylcarnitine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.21 ? x * 82000, L-CPT I, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.21 30
-
 assay at Sus scrofa