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Literature summary extracted from

  • Kashfi, K.; Mynatt, R.L.; Cook, G.A.
    Hepatic carnitine palmitoyltransferase-I has two independent inhibitory binding sites for regulation of fatty acid oxidation (1994), Biochim. Biophys. Acta, 1212, 245-252.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 4-hydroxy phenylglyoxylate CPT I, greatly reduced inhibition of protease treated enzyme Rattus norvegicus
2.3.1.21 acetyl-CoA CPT I, inhibition is not affected by proteinase treatment Rattus norvegicus
2.3.1.21 carnitine CPT I, slightly Rattus norvegicus
2.3.1.21 CoA CPT I, inhibition is not affected by proteinase treatment Rattus norvegicus
2.3.1.21 Hemipalmitoylcarnitinium bromide CPT I, strong, active-site-directed, inhibition not altered by protease treatment; i.e. HPC, 2S,6R:2R,6S-6-carboxymethyl-2-hydroxy-2-pentadecyl-4,4-dimethylmorpholinium bromide Rattus norvegicus
2.3.1.21 malonyl-CoA partial proteolysis of CPT I greatly diminishes the inhibitory effect Rattus norvegicus
2.3.1.21 methylmalonyl-CoA partial proteolysis of CPT I slightly diminishes the inhibitory effect Rattus norvegicus
2.3.1.21 additional information CPT I, 2 independent binding sites for acyl-CoA inhibitors and regulation of fatty acid oxidation, the malonyl-CoA site is located on the cytoplasmic face of the outer mitochondrial membrane, the site for monocarboxylic acids and free CoA is located in the mitochondrial intermembrane space Rattus norvegicus
2.3.1.21 palmitoyl-CoA CPT I, slightly Rattus norvegicus
2.3.1.21 propionyl-CoA CPT I, inhibition is not affected by proteinase treatment Rattus norvegicus
2.3.1.21 Ro 25-0187 CPT I, strong, inhibitory effect is drastically reduced by protease treatment of outer mitochondrial membrane; malonyl-CoA analogue Rattus norvegicus
2.3.1.21 succinyl-CoA partial proteolysis of CPT I slightly diminishes the inhibitory effect Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.21 mitochondrion CPT I Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine Rattus norvegicus CPT I, involved in regulation of fatty acid oxidation CoA + L-acylcarnitine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Rattus norvegicus
-
 enzyme form CPT I
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.21 palmitoyl-CoA + L-carnitine = CoA + L-palmitoylcarnitine 2 independent inhibitory sites Rattus norvegicus
a
2.3.1.21 palmitoyl-CoA + L-carnitine = CoA + L-palmitoylcarnitine CPT I acts at 2 distinct sites, a regulatory and an active site Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.21 liver CPT I Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.21 0.009
-
 CPT I Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-acylcarnitine
-
 r
2.3.1.21 acyl-CoA + L-carnitine CPT I, involved in regulation of fatty acid oxidation Rattus norvegicus CoA + L-acylcarnitine
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-palmitoylcarnitine
-
 r