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Literature summary extracted from

  • Akao, T.; Kusaka, T.
    Solubilization of diglyceride acyltransferase from the membrane of Mycobacterium smegmatis (1976), J. Biochem., 80, 723-728.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.20 antichaotropic salts stabilize solubilized enzyme Mycolicibacterium smegmatis
2.3.1.20 asolectin, GSH, DTT do not stabilize Mycolicibacterium smegmatis
2.3.1.20 phosphate buffer, high concentration, stabilizes Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 5,5'-dithio-bis(2-nitrobenzoic acid) i.e. DTNB, complete inhibition, DTT protects Mycolicibacterium smegmatis
2.3.1.20 cholate
-
Mycolicibacterium smegmatis
2.3.1.20 deoxycholate
-
Mycolicibacterium smegmatis
2.3.1.20 additional information diethyl-p-nitrophenylphosphate, diisopropyl fluorophosphate Mycolicibacterium smegmatis
2.3.1.20 N-ethylmaleimide inhibits solubilized enzyme, but not membrane-bound enzyme Mycolicibacterium smegmatis
2.3.1.20 p-chloromercuribenzene sulfonate complete inhibition, DTT protects Mycolicibacterium smegmatis
2.3.1.20 p-chloromercuribenzoate complete inhibition, DTT protects Mycolicibacterium smegmatis
2.3.1.20 sodiumdodecyl sulfate
-
Mycolicibacterium smegmatis
2.3.1.20 Triton WR1339
-
Mycolicibacterium smegmatis
2.3.1.20 Triton X-100
-
Mycolicibacterium smegmatis
2.3.1.20 Tween 20
-
Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.20 0.006
-
palmitoyl-CoA
-
Mycolicibacterium smegmatis
2.3.1.20 0.014
-
1,2-diolein
-
Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane firmly bound Mycolicibacterium smegmatis 16020
-

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.1.20 Acetone aqueous, stable to Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 solubilized by aqueous acetone Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
2.3.1.20 0°C, inactivation after 4 days in Tris-HCl buffer Mycolicibacterium smegmatis
2.3.1.20 0°C, more than 50% of activity retained after 4 days in 0.25 M phosphate buffer Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 1,2-diolein + palmitoyl-CoA
-
Mycolicibacterium smegmatis 1,2-dioleoyl-3-palmitoyl-sn-glycerol + CoA
-
?
2.3.1.20 acyl-CoA + 1,2-diacylglycerol
-
Mycolicibacterium smegmatis CoA + triacylglycerol
-
?
2.3.1.20 palmitoyl-CoA + 1,2-diacylglycerol
-
Mycolicibacterium smegmatis CoA + 1,2-diacyl-3-palmitoylglycerol
-
?
2.3.1.20 palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
Mycolicibacterium smegmatis CoA + 1,2-dioleoyl-3-palmitoylglycerol
-
?
2.3.1.20 palmitoyl-CoA + 1,2-dipalmitin
-
Mycolicibacterium smegmatis CoA + tripalmitin
-
?
2.3.1.20 palmitoyl-CoA + 1,2-distearin
-
Mycolicibacterium smegmatis CoA + 3-palmitoyl-1,2-distearoylglycerol
-
?
2.3.1.20 palmitoyl-CoA + 1,3-diolein
-
Mycolicibacterium smegmatis CoA + 1,3-dioleoyl-2-palmitoylglycerol
-
?
2.3.1.20 palmitoyl-CoA + 1,3-dipalmitin
-
Mycolicibacterium smegmatis CoA + tripalmitin
-
?
2.3.1.20 palmitoyl-CoA + 1-monoolein
-
Mycolicibacterium smegmatis CoA + ?
-
?
2.3.1.20 palmitoyl-CoA + 1-monopalmitin
-
Mycolicibacterium smegmatis CoA + ?
-
?
2.3.1.20 palmitoyl-CoA + 1-monostearin
-
Mycolicibacterium smegmatis CoA + ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 8 9
-
Mycolicibacterium smegmatis