Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bell, R.M.; Miller, Y.
    A highly sensitive assay for microsomal diacyglycerol acyltransferase activity from isolated fat cells (1976), Anal. Biochem., 71, 436-443.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 bovine serum albumin 65% activation at 1 mg/ml, microsomal preparation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 EDTA
-
 Rattus norvegicus
2.3.1.20 ethanol 43% inhibition when 0.04 ml are added to reaction mixture Rattus norvegicus
2.3.1.20 Tween 20 99% inhibition at 5 mg/ml, 89% inhibition at 1 mg/ml, 49% inhibition at 0.5 mg/ml Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+ 2.4fold at 8 mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol Rattus norvegicus last reaction in triacylglycerol synthesis CoA + triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Rattus norvegicus
-
 female Charles River CD
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 adipocyte
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.20 0.05
-
-
 Rattus norvegicus
2.3.1.20 0.05
-
 above Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacylglycerol last reaction in triacylglycerol synthesis Rattus norvegicus CoA + triacylglycerol
-
 ?
2.3.1.20 palmitoyl-CoA + 1,2-diacylglycerol diacylglycerol diluted in ethanol Rattus norvegicus CoA + 1,2-diacyl-3-palmitoylglycerol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 additional information
-
 assay at various temperatures from 24°C to 40°C Rattus norvegicus
2.3.1.20 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 8
-
 microsomal preparation Rattus norvegicus