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Literature summary extracted from

  • Liu, S.J.; Steinbuchel, A.
    Exploitation of butyrate kinase and phosphotransbutyrylase from Clostridium acetobutylicum for the in vitro biosynthesis of poly(hydroxyalkanoic acid) (2000), Appl. Microbiol. Biotechnol., 53, 545-552.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.19 biotechnology use of enzyme for in vitro biosynthesis of poly(hydroxyalkanoic acid) Clostridium acetobutylicum
2.7.2.7 synthesis butanoate kinase and phosphotransbutyrylase are successfully exploited for in vitro synthesis of 3-hydroxybutyryl-CoA, 4-hydroxybutyryl-CoA, 4-hydroxyvaleryl-CoA and poly(hydroxyalkanoic acid). Combination of butanoate kinase, phosphotransbutyrylase and poly(hydroxyalkanoic acid) synthase of Chromatium vinosum establishes a new system for in vitro synthesis of poly(3-hydroxybutyric acid) Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.19 Clostridium acetobutylicum
-
 recombinant enzyme
-
2.7.2.7 Clostridium acetobutylicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.19 recombinant enzyme Clostridium acetobutylicum
2.7.2.7
-
 Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.19 3-hydroxybutanoyl-CoA + phosphate
-
 Clostridium acetobutylicum 3-hydroxybutanoylphosphate + CoA
-
 r
2.3.1.19 4-hydroxybutanoyl-CoA + phosphate
-
 Clostridium acetobutylicum 4-hydroxybutanoylphosphate + CoA
-
 r
2.3.1.19 4-hydroxypentanoyl-CoA + phosphate
-
 Clostridium acetobutylicum 4-hydroxypentanoylphosphate + CoA
-
 r
2.3.1.19 butanoyl-CoA + phosphate
-
 Clostridium acetobutylicum butanoylphosphate + CoA
-
 r
2.7.2.7 ATP + butanoate
-
 Clostridium acetobutylicum ADP + butanoyl phosphate
-
 ?