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Literature summary extracted from

  • Spencer, J.B.; Jordan, P.M.
    Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum (1992), Biochem. J., 288, 839-846.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.165 PMSF and benzamidine in disruption buffer extend half-life from 20 min to 6 h, 15% glycerol and proteinase inhibitors further improve stability Penicillium griseofulvum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.165 1,3-dibromo-propane-2-one 0.02 mM, complete inactivation after 40 min, acetyl-CoA protects Penicillium griseofulvum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.165 0.007
-
 malonyl-CoA
-
 Penicillium griseofulvum
2.3.1.165 0.01
-
 acetyl-CoA
-
 Penicillium griseofulvum
2.3.1.165 0.012
-
 NADPH
-
 Penicillium griseofulvum
2.3.1.165 0.065
-
 acetoacetyl-CoA
-
 Penicillium griseofulvum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.165 180000
-
 alpha4, 4 * 180000, SDS-PAGE Penicillium griseofulvum
2.3.1.165 750000
-
 gel filtration Penicillium griseofulvum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.165 Penicillium griseofulvum
-
 NRRL 2159A
-
2.3.1.165 Penicillium griseofulvum
-
 also known as Penicillium urticae
-
2.3.1.165 Penicillium griseofulvum NRRL 2159A
-
 NRRL 2159A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.165 ammonium sulfate, poly(ethylene glycol) 6000, DEAE-Sepharose, hydroxyapatite, Mono Q Penicillium griseofulvum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.165 acetyl-CoA + 3 malonyl-CoA + NADPH + H+ = 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O a multienzyme complex with a 4'-phosphopantetheine prosthetic group on the acyl carrier protein, it has a similar sequence to vertebrate type I fatty acid synthase, acetoacetyl-CoA can also act as a starter molecule Penicillium griseofulvum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.165 0.245
-
-
 Penicillium griseofulvum

Storage Stability

EC Number Storage Stability Organism
2.3.1.165 -70°C, ammonium sulfate precipitate, loss of 5% activity per month Penicillium griseofulvum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.165 acetoacetyl-CoA + malonyl-CoA + NADPH
-
 Penicillium griseofulvum ?
-
 ?
2.3.1.165 acetoacetyl-CoA + malonyl-CoA + NADPH
-
 Penicillium griseofulvum NRRL 2159A ?
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH Penicillium griseofulvum triacetic lactone
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH Penicillium griseofulvum NRRL 2159A triacetic lactone
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH
-
 Penicillium griseofulvum 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?
2.3.1.165 acetyl-CoA + malonyl-CoA + NADPH
-
 Penicillium griseofulvum NRRL 2159A 6-methylsalicylate + CoA + CO2 + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.165 tetramer alpha4, 4 * 180000, SDS-PAGE Penicillium griseofulvum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.165 7.6
-
-
 Penicillium griseofulvum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.165 6.5 9
-
 Penicillium griseofulvum