Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schulz, H.; Staack, H.
    3-Ketoacyl-Co-A-thiolase with broad chain length specificity from pig heart muscle (1981), Methods Enzymol., 71, 389-403.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.16 glycerol or 2-mercaptoethanol stabilizes Sus scrofa
2.3.1.16 inactivation at Tris-HCl concentration Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.16 iodoacetamide
-
Sus scrofa
2.3.1.16 long-chain 3-oxoacyl-CoA compounds
-
Sus scrofa
2.3.1.16 N-Methylmaleimide
-
Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.16 0.0021
-
3-oxodecanoyl-CoA
-
Sus scrofa
2.3.1.16 0.0024
-
3-oxooctanoyl-CoA
-
Sus scrofa
2.3.1.16 0.0083
-
3-oxohexanoyl-CoA
-
Sus scrofa
2.3.1.16 0.0087
-
CoA-SH with acetoacetyl-CoA Sus scrofa
2.3.1.16 0.017
-
acetoacetyl-CoA
-
Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.16 mitochondrion
-
Sus scrofa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.16 46000
-
4 * 46000, SDS-PAGE Sus scrofa
2.3.1.16 200000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.16 CoA + 3-oxoacyl-CoA Sus scrofa enzyme catalyses the last step in the beta-oxidation cycle acyl-CoA + acetyl-CoA
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.16 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.16
-
Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.16 heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.16 additional information
-
assay method Sus scrofa
2.3.1.16 597
-
-
Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.3.1.16 25°C, 0.75 M Tris-HCl, pH 8.1, 25% v/v glycerol, 10 mM 2-mercaptoethanol, stable for days Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.16 CoA + 3-oxoacyl-CoA
-
Sus scrofa acyl-CoA + acetyl-CoA
-
r
2.3.1.16 CoA + 3-oxoacyl-CoA enzyme catalyses the last step in the beta-oxidation cycle Sus scrofa acyl-CoA + acetyl-CoA
-
r
2.3.1.16 CoA + 3-oxodecanoyl-CoA
-
Sus scrofa acetyl-CoA + octanoyl-CoA
-
r
2.3.1.16 CoA + 3-oxohexanoyl-CoA
-
Sus scrofa acetyl-CoA + butanoyl-CoA
-
r
2.3.1.16 CoA + 3-oxooctanoyl-CoA
-
Sus scrofa acetyl-CoA + hexanoyl-CoA
-
r
2.3.1.16 CoA + acetoacetyl-CoA
-
Sus scrofa acetyl-CoA + acetyl-CoA
-
r
2.3.1.16 additional information acetoacetyl-CoA is a poor substrate Sus scrofa ?
-
?
2.3.1.16 additional information acts on 3-oxoacyl-CoA compounds of various chain lengths Sus scrofa ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.16 tetramer 4 * 46000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.16 25
-
pH 8.1, 25% glycerol v/v, stable for days Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.16 7.6
-
-
Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.16 7.6 8.3 pH 7.6: maximum activity, pH 8.3: about 75% of maximum activity Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.16 8.1
-
25°C, 25% glycerol v/v, stable for days Sus scrofa