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Literature summary extracted from

  • Lukacin, R.; Schreiner, S.; Matern, U.
    Transformation of acridone synthase to chalcone synthase (2001), FEBS Lett., 508, 413-417.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74
-
 Ruta graveolens
2.3.1.159 expression of wild-type and mutants in Escherichia coli Ruta graveolens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.74 A133S fully functional enzyme Ruta graveolens
2.3.1.74 S132T fully functional enzyme Ruta graveolens
2.3.1.74 V265F reduced activity Ruta graveolens
2.3.1.74 V265F/S132T/A133S triple mutant, reduced specific activity Ruta graveolens
2.3.1.159 S132T/A133S/V265F site-directed mutagenesis, exchange of 3 residues, responsible for substrate activity, by the corresponding amino acids of chalcone synthase results in transformation of the enzyme to a chalcone synthase, 25fold increased activity, with only 36% remaining acridone synthase activity compared to wild-type Ruta graveolens
2.3.1.159 V265F site-directed mutagenesis, 75% reduction of ACS catalytic activity, but 2fold increased chalcone synthase activity Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.159 additional information
-
 structure model of isozyme II Ruta graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens
-
 CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Ruta graveolens
-
-
-
2.3.1.159 Ruta graveolens
-
 2 isozymes I and II
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.159 3 malonyl-CoA + N-methylanthraniloyl-CoA = 4 CoA + 1,3-dihydroxy-N-methylacridone + 3 CO2 + H2O two-step mechanism Ruta graveolens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.159 malonyl-CoA + 4-coumaroyl-CoA wild-type shows low activity, while mutants show highly increased activity Ruta graveolens CoA + naringenin chalcone
-
 ?
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA
-
 Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.3.1.159 ACS
-
 Ruta graveolens