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Literature summary extracted from

  • Junghanns, K.T.; Kneusel, R.E.; Baumert, A.; Maier, W.; Groeger, D.; Matern, U.
    Molecular cloning and heterologous expression of acridone synthase from elicited Ruta graveolens L. cell suspension cultures (1995), Plant Mol. Biol., 27, 681-692.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.159 expression in Escherichia coli strain BL21 (DE3)pLys S and in lambda phages via Escherichia coli host, DNA sequence determination Ruta graveolens
2.3.1.159 expression level in Escherichia coli is decreased by light irradiation and increased by treatment with elicitors from the cell wall of Phytophthora megasperma f. sp. glycinea Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.159 42000
-
 1 * 42000, recombinant enzyme from Escherichia coli, SDS-PAGE and western blot analysis Ruta graveolens
2.3.1.159 44000
-
 recombinant enzyme from E. coli, gel filtration Ruta graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens activity is increased upon treatment with crude elicitors from the cell wall of Phytophthora megasperma f. sp. glycinea, syn. Phytophthora sojae, and decreased upon light irradiation CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.159 Ruta graveolens Q9FSC0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.159 cell suspension culture
-
 Ruta graveolens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA
-
 Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ir
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA activity is increased upon treatment with crude elicitors from the cell wall of Phytophthora megasperma f. sp. glycinea, syn. Phytophthora sojae, and decreased upon light irradiation Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ir

Subunits

EC Number Subunits Comment Organism
2.3.1.159 monomer 1 * 42000, recombinant enzyme from Escherichia coli, SDS-PAGE and western blot analysis Ruta graveolens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.159 ACS
-
 Ruta graveolens