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Literature summary extracted from

  • Takamura, Y.; Kitayama, Y.
    Purification and some properties of malonate decarboxylase from Pseudomonas ovalis: an oligomeric enzyme with bifunctional properties (1981), Biochem. Int., 3, 483-491.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.9 additional information
-
 additional information
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.3 30000
-
 1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida
2.8.3.3 40000
-
 1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida
2.8.3.3 70000
-
 1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida
2.8.3.3 170000
-
 gel filtration Pseudomonas putida
4.1.1.9 30000
-
 x * 30000 + x * 40000 + x * 70000, SDS-PAGE Pseudomonas putida
4.1.1.9 40000
-
 x * 30000 + x * 40000 + x * 70000, SDS-PAGE Pseudomonas putida
4.1.1.9 70000
-
 x * 30000 + x * 40000 + x * 70000, SDS-PAGE Pseudomonas putida
4.1.1.9 170000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.3 acetyl-CoA + malonate Pseudomonas putida inducible enzyme acetate + malonyl-CoA
-
 ?
2.8.3.3 acetyl-CoA + malonate Pseudomonas putida IAM 1177 inducible enzyme acetate + malonyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.3 Pseudomonas putida
-
 IAM 1177
-
2.8.3.3 Pseudomonas putida IAM 1177
-
 IAM 1177
-
4.1.1.9 Pseudomonas putida
-
 bifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.3 partial Pseudomonas putida
4.1.1.9
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.3 acetyl-CoA + malonate absolutely dependent on acetyl-CoA Pseudomonas putida acetate + malonyl-CoA
-
 ?
2.8.3.3 acetyl-CoA + malonate inducible enzyme Pseudomonas putida acetate + malonyl-CoA
-
 ?
2.8.3.3 acetyl-CoA + malonate absolutely dependent on acetyl-CoA Pseudomonas putida IAM 1177 acetate + malonyl-CoA
-
 ?
2.8.3.3 acetyl-CoA + malonate inducible enzyme Pseudomonas putida IAM 1177 acetate + malonyl-CoA
-
 ?
2.8.3.3 butyryl-CoA + malonate
-
 Pseudomonas putida malonyl-CoA + butanoate
-
 ?
2.8.3.3 malonyl-CoA + malonate exchange reaction Pseudomonas putida malonyl-CoA + malonate
-
 ?
2.8.3.3 methylmalonyl-CoA + malonate
-
 Pseudomonas putida malonyl-CoA + methylmalonate
-
 ?
2.8.3.3 additional information succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA Pseudomonas putida ?
-
 ?
2.8.3.3 additional information succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA Pseudomonas putida IAM 1177 ?
-
 ?
2.8.3.3 propionyl-CoA + malonate
-
 Pseudomonas putida malonyl-CoA + propanoate
-
 ?
4.1.1.9 Malonyl-CoA
-
 Pseudomonas putida Acetyl-CoA + CO2
-
 ?
4.1.1.9 Malonyl-CoA
-
 Pseudomonas putida IAM 1177 Acetyl-CoA + CO2
-
 ?
4.1.1.9 methylmalonyl-CoA
-
 Pseudomonas putida propionyl-CoA + CO2
-
 ?
4.1.1.9 methylmalonyl-CoA
-
 Pseudomonas putida IAM 1177 propionyl-CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.3 tetramer 1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida
4.1.1.9 ? x * 30000 + x * 40000 + x * 70000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.3 30
-
 assay at Pseudomonas putida