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Literature summary extracted from

  • Zheng, Z.; Zou, J.
    The initial step of the glycerolipid pathway: identification of glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae (2001), J. Biol. Chem., 276, 41710-41716.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 expression in Escherichia coli Saccharomyces cerevisiae
2.3.1.42 genes GAT1 and GAT2, expression in Escherichia coli DH5alpha, Gat1 and Gat2 proteins are glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferases, amino acid sequence analysis Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.42 additional information null mutations of GAT1 andGAT2 are lethal Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Saccharomyces cerevisiae P32784 Gat2p
-
2.3.1.15 Saccharomyces cerevisiae P36148 Gat1p
-
2.3.1.42 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.42 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate enzyme has both 3-glycerophosphate acyltransferase and dihydroxyacetone phosphate acyltransferase activity Saccharomyces cerevisiae CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Saccharomyces cerevisiae CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate low activity Saccharomyces cerevisiae CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
 Saccharomyces cerevisiae CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Saccharomyces cerevisiae CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate preferred acyl-donor Saccharomyces cerevisiae CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Saccharomyces cerevisiae CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate low activity Saccharomyces cerevisiae CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.42 acyl-CoA + dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae CoA + acyldihydroxyacetone phosphate
-
 ?
2.3.1.42 cis-9-hexadecenoyl-CoA + dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae CoA + cis-9-hexadecenoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 additional information Gat1 and Gat2 proteins are glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferases Saccharomyces cerevisiae ?
-
 ?
2.3.1.42 octadecenoyl-CoA + dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae CoA + heptadecenoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 stearoyl-CoA + dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae CoA + stearoyldihydroxyacetone phosphate
-
 ?