EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.15 | Phospholipids | absolutely required | Escherichia coli | |
2.3.1.15 | Phospholipids | bilayers required for activity: phosphatidylethanolamine/-glycerol, ratio 4/1, phosphatidylethanolamine/cardiolipin, ratio 4/1 | Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
2.3.1.15 | 400 mM NaCl or KCl, 5 mM 2-mercaptoethanol, 5 mM MgCl2, stabilize membrane-bound or reconstituted enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.15 | 0.05 | - |
sn-glycerol 3-phosphate | acyl-CoA | Escherichia coli | |
2.3.1.15 | 0.07 | - |
acyl-ACP | - |
Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.15 | plasma membrane | - |
Escherichia coli | 5886 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.15 | Escherichia coli | - |
K-12 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.15 | partial purification | Escherichia coli |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.3.1.15 | reconstituted at a phospholipid-detergent ratio of 20:1 | Escherichia coli |
2.3.1.15 | reconstitution in vesicles | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.15 | 0.105 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.15 | -20°C, at least 6 months | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.15 | acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | acyl-CoA + sn-glycerol 3-phosphate | dihydroxyacetone phosphate is ineffective as acyl-acceptor | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | acyl-CoA + sn-glycerol 3-phosphate | saturated acyl-CoAs preferred | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.15 | 8.5 | - |
- |
Escherichia coli |