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Literature summary extracted from

  • Kessels, J.M.M.; Ousen, H.; Van Den Bosch, H.
    Studies on reconstituted partially purified glycerophosphate acyltransferase from Escherichia coli (1984), Eur. J. Biochem., 138, 543-549.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 Phospholipids
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 Monoacylglycerophosphate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 mitochondrial inner membrane
-
 Escherichia coli 5743
-
2.3.1.15 plasma membrane
-
 Escherichia coli 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 solubilized Escherichia coli
2.3.1.15 solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.15 an acyl-CoA + sn-glycerol 3-phosphate = CoA + a 1-acyl-sn-glycerol 3-phosphate mechanism Escherichia coli
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstitution in phosphatidylcholine/phosphatidylglycerol, ratio 4:1, vesicles, 90% of active site of the reconstituted enzyme are located at the outside surface Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate temperature-dependent specificity of reconstituted enzyme Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate saturated acyl-CoAs preferred Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate i.e. n-hexadecanoyl-CoA, best substrate Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 30
-
 reconstituted enzyme Escherichia coli