Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kessels, J.M.M.; Van Den Bosch, H.
    Characterization of reconstituted partially purified glycerophosphate acyltansferase from Escherichia coli (1982), Biochim. Biophys. Acta, 713, 570-580.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 phosphatidylglycerol
-
Escherichia coli
2.3.1.15 Phospholipids
-
Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.15 ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography, gel filtration and isoelectric focusing inactivate enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 Triton X-100
-
Escherichia coli
2.3.1.15 Trypsin
-
Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 plasma membrane active site on outside surface Escherichia coli 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Escherichia coli
-
CoA + 1-acyl-sn-glycerol 3-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Escherichia coli
-
K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 partial purification Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstitution in phosphatidylcholine/phosphatidylglycerol, ratio 4:1, vesicles, 90% of active site of the reconstituted enzyme are located at the outside surface Escherichia coli
2.3.1.15 reconstitution in vesicles Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.015
-
reconstituted enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.15 -40°C, solubilized enzyme, at least 1 year, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 20
-
reconstituted enzyme, 2 optima Escherichia coli
2.3.1.15 30 35 membrane-associated enzyme Escherichia coli
2.3.1.15 35
-
reconstituted enzyme, 2 optima Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.15 10 50 approx. half-maximal activity at 10°C and 50°C Escherichia coli
2.3.1.15 20 40 approx. half-maximal activity at 20°C and about 70% of maximal activity at 40°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 9.2
-
reconstituted enzyme Escherichia coli
2.3.1.15 9.5
-
membrane-associated enzyme Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.15 7.5 9.2 approx. half-maximal activity at pH 7.5 and 9.2, reconstituted enzyme Escherichia coli
2.3.1.15 8 9.5 approx. half-maximal activity at pH 8.0 and 9.5, membrane-associated enzyme Escherichia coli