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Literature summary extracted from

  • Bertrams, M.; Heinz, E.
    Positional specificity and fatty acid selectivity of purified sn-glycerol 3-phosphate acyltransferases from chloroplasts (1981), Plant Physiol., 68, 653-657.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 (3-Morpholino)propanesulfonic acid-NaOH i.e. MOPS-NaOH, activation, up to 30 mM Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.7
-
 sn-glycerol 3-phosphate isoforms 1 and 2 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 chloroplast soluble Spinacia oleracea 9507
-
2.3.1.15 chloroplast soluble Pisum sativum 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 additional information not activated by divalent ions Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 42000
-
 2 soluble chloroplast isoforms, gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Pisum sativum
-
 pea, var. kleine Rheinländerin
-
2.3.1.15 Spinacia oleracea
-
 var. Vital
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15
-
 Spinacia oleracea
2.3.1.15 2 isoforms of different pI-values Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 leaf
-
 Spinacia oleracea
-
2.3.1.15 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.015
-
-
 Spinacia oleracea
2.3.1.15 1.74
-
 isoform 1 Pisum sativum
2.3.1.15 3.2
-
 isoform 2 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.3.1.15 -20°C, 50% w/v glycerol, 3 years, no loss of activity Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate chloroplastic enzyme has no strict acyl-CoA specificity Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate chloroplastic enzyme has no strict acyl-CoA specificity Pisum sativum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate specific for glycerol 3-phosphate Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate specific for glycerol 3-phosphate Pisum sativum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Pisum sativum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate dihydroxyacetone phosphate is ineffective as acyl-acceptor Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate dihydroxyacetone phosphate is ineffective as acyl-acceptor Pisum sativum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 linolenoyl-CoA + sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-linolenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 linolenoyl-CoA + sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-linolenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 linoleoyl-CoA + sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-linoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 linoleoyl-CoA + sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-linoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate preferred substrate Pisum sativum CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate preferred in an equimolar mixture of palmitoyl-CoA, stearoyl-CoA and oleoyl-CoA Spinacia oleracea CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate preferred in an equimolar mixture of palmitoyl-CoA, stearoyl-CoA and oleoyl-CoA Pisum sativum CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 6.6
-
 2 isoforms Pisum sativum
2.3.1.15 7.4
-
 isoforms 1 and 2 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.15 acyl-CoA activation, above 0.02 mM, in the presence of bovine serum albumin Pisum sativum