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Literature summary extracted from

  • Kasinathan, C.; Grzelinska, E.; Okazaki, K.; Slomiany, B.L.; Slomiany, A.
    Purification of protein fatty acyltransferase and determination of its distribution and topology (1990), J. Biol. Chem., 265, 5139-5144.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.142 dithiothreitol activation Rattus norvegicus
2.3.1.142 Triton X-100 activation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.142 glycerol, 25% v/v, stabilizes Rattus norvegicus
2.3.1.142 protease inhibitors stabilize Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.142 EDTA
-
 Rattus norvegicus
2.3.1.142 MgCl2
-
 Rattus norvegicus
2.3.1.142 MnCl2
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.142 microsome rough microsomes, integral part of rough endoplasmic reticulum, exposed to cytoplasmic side Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.142 NaF activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.142 65000
-
 x * 65000 + x * 67000, SDS-PAGE Rattus norvegicus
2.3.1.142 67000
-
 x * 65000 + x * 67000, SDS-PAGE Rattus norvegicus
2.3.1.142 243000
-
 native PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.142 palmitoyl-CoA + mucus glycoprotein Rattus norvegicus involved in modification of membrane glycoproteins CoA + O-palmitoylglycoprotein
-
 ?
2.3.1.142 palmitoyl-CoA + mucus glycoprotein Rattus norvegicus Sprague-Dawley involved in modification of membrane glycoproteins CoA + O-palmitoylglycoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.142 Rattus norvegicus
-
 Sprague-Dawley
-
2.3.1.142 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.142 gastric mucosa, solubilized with Triton X-100, affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.142 gastric mucosa
-
 Rattus norvegicus
-
2.3.1.142 heart
-
 Rattus norvegicus
-
2.3.1.142 kidney
-
 Rattus norvegicus
-
2.3.1.142 liver
-
 Rattus norvegicus
-
2.3.1.142 lung
-
 Rattus norvegicus
-
2.3.1.142 lymphoblast
-
 Rattus norvegicus
-
2.3.1.142 pancreas
-
 Rattus norvegicus
-
2.3.1.142 salivary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.142 0.000934
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.142 -80°C, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.142 palmitoyl-CoA + mucus glycoprotein
-
 Rattus norvegicus CoA + O-palmitoylglycoprotein
-
 ?
2.3.1.142 palmitoyl-CoA + mucus glycoprotein involved in modification of membrane glycoproteins Rattus norvegicus CoA + O-palmitoylglycoprotein
-
 ?
2.3.1.142 palmitoyl-CoA + mucus glycoprotein
-
 Rattus norvegicus Sprague-Dawley CoA + O-palmitoylglycoprotein
-
 ?
2.3.1.142 palmitoyl-CoA + mucus glycoprotein involved in modification of membrane glycoproteins Rattus norvegicus Sprague-Dawley CoA + O-palmitoylglycoprotein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.142 ? x * 65000 + x * 67000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.142 37
-
 assay at Rattus norvegicus