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Literature summary extracted from

  • Hohlfeld, M.; Veit, M.; Strack, D.
    Hydroxycinnamoyltransferases Involved in the accumulation of caffeic acid esters in gametophytes and sporophytes of Equisetum arvense (1996), Plant Physiol., 111, 1153-1159.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.133 0.41
-
caffeoyl-CoA
-
Equisetum arvense
2.3.1.133 0.5
-
4-coumaroyl-CoA
-
Equisetum arvense
2.3.1.133 1.1
-
3-phenylacryloyl-CoA
-
Equisetum arvense
2.3.1.133 1.9
-
feruloyl-CoA
-
Equisetum arvense
2.3.1.133 4.32
-
shikimate
-
Equisetum arvense
2.3.1.133 5.5
-
shikimate
-
Equisetum arvense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.133 50000
-
gel filtration Equisetum arvense

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Equisetum arvense
-
-
-
2.3.1.133 Equisetum arvense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.133 partial Equisetum arvense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 additional information
-
change of enzyme activity during a 1-year growth period Equisetum arvense
2.3.1.133 additional information
-
change of enzyme activity during a 1-year growth period Equisetum arvense

Storage Stability

EC Number Storage Stability Organism
2.3.1.133 -20°C, for several months with 10-20% loss of apparent activities after thawing Equisetum arvense
2.3.1.133 -80°C, for several months with 10-20% loss of apparent activities after thawing Equisetum arvense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 caffeoyl-CoA + quinate
-
Equisetum arvense CoA + 5-O-caffeoylquinate i.e. chlorogenic acid ?
2.3.1.133 3-phenylacryloyl-CoA + shikimate
-
Equisetum arvense CoA + 3-phenylacryloylshikimate
-
?
2.3.1.133 4-coumaroyl-CoA + shikimate shikimate cannot be replaced by quinate Equisetum arvense CoA + 5-O-(4-coumaroyl)shikimate
-
?
2.3.1.133 caffeoyl-CoA + shikimate
-
Equisetum arvense CoA + 5-O-caffeoylshikimate
-
?
2.3.1.133 cinnamoyl-CoA + shikimate
-
Equisetum arvense CoA + 5-O-cinnamoylshikimate
-
?
2.3.1.133 feruloyl-CoA + shikimate
-
Equisetum arvense CoA + 5-O-feruloylshikimate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.133 40
-
-
Equisetum arvense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.133 7.5
-
Tris-HCl Equisetum arvense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.133 6.2 8.5 about half-maximal activity at pH 6.2 in potassium phosphate buffer and 8.5 in Tris-HCl puffer Equisetum arvense