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Literature summary extracted from

  • Lotfy, L.; Fleuriet, A.; Macheix, J.J.
    Partial purification and characterization of hydroxycinnamoyl CoA:transferase from apple and date fruits (1992), Phytochemistry, 31, 767-772.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.133 glycerol stabilizes during storage Phoenix dactylifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.99 Sodium diphosphate
-
 Malus sylvestris
2.3.1.99 tricine
-
 Malus sylvestris
2.3.1.99 Tris/HCl reversible Malus sylvestris
2.3.1.133 Sodium diphosphate
-
 Phoenix dactylifera
2.3.1.133 tricine weak, not Tris/HCl Phoenix dactylifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 0.008
-
 coumaroyl-CoA
-
 Malus sylvestris
2.3.1.99 0.009
-
 feruloyl-CoA
-
 Malus sylvestris
2.3.1.99 0.01
-
 caffeoyl-CoA
-
 Malus sylvestris
2.3.1.99 1.3
-
 quinate
-
 Malus sylvestris
2.3.1.99 10
-
 shikimate
-
 Malus sylvestris
2.3.1.133 0.006
-
 feruloyl-CoA
-
 Phoenix dactylifera
2.3.1.133 0.0067
-
 caffeoyl-CoA
-
 Phoenix dactylifera
2.3.1.133 0.0086
-
 4-coumaroyl-CoA
-
 Phoenix dactylifera
2.3.1.133 1
-
 shikimate
-
 Phoenix dactylifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.99 40000
-
 gel filtration Malus sylvestris
2.3.1.133 40000
-
 gel filtration Phoenix dactylifera

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Malus sylvestris
-
 apple, cv. Golden Delicious
-
2.3.1.133 Phoenix dactylifera
-
 young green dates
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.99 partial Malus sylvestris
2.3.1.133 partial Phoenix dactylifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.99 fruit
-
 Malus sylvestris
-
2.3.1.133 fruit
-
 Phoenix dactylifera
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 0.37
-
-
 Malus sylvestris
2.3.1.133 0.732
-
-
 Phoenix dactylifera

Storage Stability

EC Number Storage Stability Organism
2.3.1.99 -20°C, with 10% glycerol at least 30 days Malus sylvestris
2.3.1.133 -20°C, with 10% glycerol at least 30 days Phoenix dactylifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 4-coumaroyl-CoA + quinate best substrate Malus sylvestris CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 4-coumaroyl-CoA + quinate no substrates are citrate, malate, L-(+)-tartrate, UDP-glucose, myo-inositol, tyramine, agmatine Malus sylvestris CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 4-coumaroyl-CoA + quinate cinnamoyl-CoA cannot replace coumaroyl-CoA Malus sylvestris CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 4-coumaroyl-CoA + quinate not: glucose Malus sylvestris CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 caffeoyl-CoA + quinate
-
 Malus sylvestris CoA + 5-O-caffeoylquinate
-
 ?
2.3.1.99 feruloyl-CoA + quinate
-
 Malus sylvestris CoA + O-feruloylquinate
-
 ?
2.3.1.99 p-coumaroyl-CoA + shikimate poor substrate Malus sylvestris CoA + 5-O-(p-coumaroyl)shikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + quinate
-
 Phoenix dactylifera CoA + 4-coumaroylquinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate best substrate Phoenix dactylifera CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Phoenix dactylifera CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.133 feruloyl-CoA + shikimate
-
 Phoenix dactylifera CoA + 5-O-feruloylshikimate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.99 45
-
-
 Malus sylvestris
2.3.1.133 40
-
-
 Phoenix dactylifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.99 additional information
-
 pI: 5.4 Malus sylvestris
2.3.1.99 6.5
-
 50 mM potassium phosphate buffer Malus sylvestris
2.3.1.133 additional information
-
 pI: 4.63 Phoenix dactylifera
2.3.1.133 6.5
-
 50 mM potassium phosphate buffer Phoenix dactylifera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.99 5.6 7.9 about half-maximal activity at pH 5.6 and 7.9 Malus sylvestris
2.3.1.133 5.5 7.5 about half-maximal activity at pH 5.5 and 7.5 Phoenix dactylifera

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.99 Malus sylvestris
-
 6.2 5.4