EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.99 | EDTA | activation | Secale cereale | |
2.3.1.130 | DTT | activation | Secale cereale | |
2.3.1.131 | EDTA | activation | Secale cereale | |
2.3.1.132 | DTT | activates | Secale cereale | |
2.3.1.133 | EDTA | activation | Secale cereale |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.99 | 0.05 | - |
feruloyl-CoA | - |
Secale cereale | |
2.3.1.99 | 4.3 | - |
quinate | - |
Secale cereale | |
2.3.1.130 | 0.045 | - |
feruloyl-CoA | - |
Secale cereale | |
2.3.1.130 | 33 | - |
galactarate | - |
Secale cereale | |
2.3.1.131 | 0.018 | - |
sinapoyl-CoA | - |
Secale cereale | |
2.3.1.131 | 8 | - |
glucarate | - |
Secale cereale | |
2.3.1.132 | 0.029 | - |
sinapoyl-CoA | - |
Secale cereale | |
2.3.1.132 | 10 | - |
glucarolactone | - |
Secale cereale | |
2.3.1.133 | 0.024 | - |
4-coumaroyl-CoA | - |
Secale cereale | |
2.3.1.133 | 0.045 | - |
caffeoyl-CoA | - |
Secale cereale | |
2.3.1.133 | 0.59 | - |
shikimate | with 4-coumaroyl-CoA | Secale cereale | |
2.3.1.133 | 83 | - |
shikimate | with caffeoyl-CoA | Secale cereale |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.132 | sinapoyl-CoA + glucarolactone | Secale cereale | best substrate | CoA + O-sinapoylglucarolactone | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.99 | Secale cereale | - |
rye, var. Kustro | - |
2.3.1.130 | Secale cereale | - |
rye, var. Kustro | - |
2.3.1.131 | Secale cereale | - |
rye, var. Kustro | - |
2.3.1.132 | Secale cereale | - |
rye, var. Kustro | - |
2.3.1.133 | Secale cereale | - |
rye, var. Kustro | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.99 | partial | Secale cereale |
2.3.1.130 | partial | Secale cereale |
2.3.1.131 | partial | Secale cereale |
2.3.1.132 | partial | Secale cereale |
2.3.1.133 | partial | Secale cereale |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.99 | leaf | primary | Secale cereale | - |
2.3.1.130 | leaf | primary | Secale cereale | - |
2.3.1.131 | leaf | primary | Secale cereale | - |
2.3.1.132 | primary leaf | - |
Secale cereale | - |
2.3.1.133 | leaf | primary | Secale cereale | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.99 | 0.0042 | - |
- |
Secale cereale |
2.3.1.130 | 0.0033 | - |
- |
Secale cereale |
2.3.1.131 | 0.01 | - |
feruloyl-CoA as donor | Secale cereale |
2.3.1.132 | 0.022 | - |
- |
Secale cereale |
2.3.1.133 | 0.0056 | - |
- |
Secale cereale |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.99 | -20°C, at least 6 months | Secale cereale |
2.3.1.130 | -20°C, at least 6 months | Secale cereale |
2.3.1.131 | -20°C, at least 6 months | Secale cereale |
2.3.1.132 | -20°C, at least 6 months | Secale cereale |
2.3.1.133 | -20°C, at least 6 months | Secale cereale |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.99 | 4-coumaroyl-CoA + quinate | at 39% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
2.3.1.99 | caffeoyl-CoA + quinate | condensation at 10% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + 5-O-caffeoylquinate | i.e. chlorogenic acid | ? | |
2.3.1.99 | feruloyl-CoA + quinate | best substrate | Secale cereale | CoA + O-feruloylquinate | - |
? | |
2.3.1.99 | p-coumaroyl-CoA + shikimate | - |
Secale cereale | CoA + 5-O-(p-coumaroyl)shikimate | - |
? | |
2.3.1.99 | sinapoyl-CoA + quinate | at 21% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + 5-O-sinapoylquinate | - |
? | |
2.3.1.130 | caffeoyl-CoA + galactarate | poor substrate | Secale cereale | CoA + O-caffeoylgalactarate | - |
? | |
2.3.1.130 | feruloyl-CoA + galactarate | best substrate, no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | CoA + O-feruloylgalactarate | - |
? | |
2.3.1.130 | p-coumaroyl-CoA + galactarate | condensation at 69% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + O-p-coumaroylgalactarate | - |
? | |
2.3.1.130 | sinapoyl-CoA + galactarate | condensation at 78% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + O-sinapoylgalactarate | - |
? | |
2.3.1.131 | caffeoyl-CoA + glucarate | condensation at 8% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + caffeoylglucarate | - |
? | |
2.3.1.131 | feruloyl-CoA + glucarate | condensation at 54% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + feruloylglucarate | - |
? | |
2.3.1.131 | additional information | no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | ? | - |
? | |
2.3.1.131 | p-coumaroyl-CoA + glucarate | condensation at 85% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + p-coumaroylglucarate | - |
? | |
2.3.1.131 | sinapoyl-CoA + glucarate | best substrate | Secale cereale | CoA + O-sinapoylglucarate | - |
? | |
2.3.1.132 | caffeoyl-CoA + glucarolactone | poor substrate | Secale cereale | CoA + O-caffeoylglucarolactone | - |
? | |
2.3.1.132 | feruloyl-CoA + glucarolactone | condensation at 37% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + O-feruloylglucarolactone | - |
? | |
2.3.1.132 | additional information | no activity with 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | ? | - |
? | |
2.3.1.132 | p-coumaroyl-CoA + glucarolactone | condensation at 54% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + O-p-coumaroylglucarolactone | - |
? | |
2.3.1.132 | sinapoyl-CoA + glucarolactone | best substrate | Secale cereale | CoA + O-sinapoylglucarolactone | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + shikimate | best substrate | Secale cereale | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
2.3.1.133 | caffeoyl-CoA + shikimate | condensation at 52% the rate of the reaction with p-coumaroyl-CoA | Secale cereale | CoA + 5-O-caffeoylshikimate | - |
? | |
2.3.1.133 | feruloyl-CoA + shikimate | condensation at 22% the rate of the reaction with p-coumaroyl-CoA | Secale cereale | CoA + 5-O-feruloylshikimate | - |
? | |
2.3.1.133 | additional information | 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | ? | - |
? | |
2.3.1.133 | sinapoyl-CoA + shikimate | condensation at 29% the rate of the reaction with p-coumaroyl-CoA | Secale cereale | CoA + 5-O-sinapoylshikimate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.130 | 30 | - |
assay at | Secale cereale |
2.3.1.131 | 30 | - |
assay at | Secale cereale |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.99 | 80 | - |
denaturation after 5 min | Secale cereale |
2.3.1.130 | 80 | - |
denaturation after 5 min | Secale cereale |
2.3.1.131 | 80 | - |
denaturation after 5 min | Secale cereale |
2.3.1.132 | 80 | - |
denaturation after 5 min | Secale cereale |
2.3.1.133 | 80 | - |
denaturation after 5 min | Secale cereale |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.99 | 7.5 | - |
100 mM potassium phosphate buffer | Secale cereale |
2.3.1.130 | 7.3 | - |
25 mM potassium phosphate buffer | Secale cereale |
2.3.1.131 | 7.3 | - |
100 mM potassium phosphate buffer | Secale cereale |
2.3.1.132 | 7.8 | - |
50 mM potassium phosphate buffer | Secale cereale |
2.3.1.133 | 7.1 | - |
200 mM potassium phosphate buffer | Secale cereale |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.99 | 6.9 | 8 | about 80% of maximal activity at pH 6.9 and 8.0 | Secale cereale |
2.3.1.130 | 7.1 | 7.6 | about 80% of maximal activity at pH 7.1 and 7.6 | Secale cereale |
2.3.1.131 | 6.2 | 8 | about 80% of maximal activity at pH 6.2 and 8.0 | Secale cereale |
2.3.1.132 | 7.4 | 8.2 | about 80% of maximal activity at pH 7.4 and pH 8.2 | Secale cereale |
2.3.1.133 | 6.3 | 7.7 | about 80% of maximal activity at pH 6.3 and 7.7 | Secale cereale |