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Literature summary extracted from

  • Webster, L.T.
    Benzoyl-CoA: amino acid and phenylacetyl-CoA: amino acid N-acyltransferases (1981), Methods Enzymol., 77, 301-308.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.13 enzyme fractions from hydroxylapatite column are inactivated by dialysis or concentration by ultracentrifugation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.71 Mg2+
-
 Bos taurus
2.3.1.71 Ni2+
-
 Bos taurus
2.3.1.71 Zn2+
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.13 mitochondrion
-
 Bos taurus 5739
-
2.3.1.71 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.13 33000
-
 SDS-PAGE, sucrose density gradient centrifugation Bos taurus
2.3.1.71 33000
-
 1 * 33000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.13 Bos taurus
-
-
-
2.3.1.71 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.13
-
 Bos taurus
2.3.1.71
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.71 benzoyl-CoA + glycine = CoA + hippurate sequential reaction mechanism in which the acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves and the peptide product dissociates last Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.13 liver
-
 Bos taurus
-
2.3.1.71 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.13 500
-
-
 Bos taurus
2.3.1.71 24
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.3.1.13 4°C, 1-3 weeks, about 25% loss of activity Bos taurus
2.3.1.71 4°C, 3 weeks, enzyme retains about 80% of its initial activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.13 acyl-CoA + glycine phenylacetyl-CoA or indoleacetyl-CoA can not act as acyl donor Bos taurus CoA + N-acylglycine
-
 ?
2.3.1.13 acyl-CoA + L-asparagine
-
 Bos taurus ?
-
 ?
2.3.1.13 acyl-CoA + L-glutamine
-
 Bos taurus ?
-
 ?
2.3.1.71 3-methylcrotonyl-CoA + glycine
-
 Bos taurus CoA + N-3-methylcrotonylglycine
-
 ?
2.3.1.71 acetyl-CoA + glycine
-
 Bos taurus CoA + N-acetylglycine
-
 ?
2.3.1.71 benzoyl-CoA + glycine
-
 Bos taurus CoA + N-benzoylglycine
-
 ?
2.3.1.71 benzoyl-CoA + L-asparagine weak activity Bos taurus CoA + N-benzoylasparagine
-
 ?
2.3.1.71 isobutyryl-CoA + glycine
-
 Bos taurus CoA + N-isobutyrylglycine
-
 ?
2.3.1.71 isovaleryl-CoA + glycine
-
 Bos taurus CoA + N-isovalerylglycine
-
 ?
2.3.1.71 n-butyryl-CoA + glycine
-
 Bos taurus CoA + N-butyrylglycine
-
 ?
2.3.1.71 propionyl-CoA + glycine
-
 Bos taurus CoA + N-propionylglycine
-
 ?
2.3.1.71 salicyl-CoA + glycine
-
 Bos taurus CoA + salicyluric acid
-
 ?
2.3.1.71 tiglyl-CoA + glycine
-
 Bos taurus CoA + N-tiglylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.71 monomer 1 * 33000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.71 8.4 8.6
-
 Bos taurus