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Literature summary extracted from

  • Tricot, C.; Vander Wauven, C.; Wattiez, R.; Falmagne, P.; Stalon, V.
    Purification and properties of a succinyltransferase from Pseudomonas aeruginosa specific for both arginine and ornithine (1994), Eur. J. Biochem., 224, 853-861.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.109 L-arginine allosteric activator of the ornithine succinyltransferase activity Pseudomonas aeruginosa
2.3.1.109 L-lysine activator of the ornithine succinyltransferase activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.109 agmatine
-
 Pseudomonas aeruginosa
2.3.1.109 D-arginine competitive inhibition Pseudomonas aeruginosa
2.3.1.109 spermidine
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.109 0.04
-
 L-ornithine
-
 Pseudomonas aeruginosa
2.3.1.109 0.5
-
 L-arginine
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.109 35000
-
 2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE Pseudomonas aeruginosa
2.3.1.109 37000
-
 2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE Pseudomonas aeruginosa
2.3.1.109 140000
-
 PAGE Pseudomonas aeruginosa
2.3.1.109 150000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.109 succinyl-CoA + L-arginine Pseudomonas aeruginosa
-
 CoA + N2-succinyl-L-arginine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.71 Pseudomonas aeruginosa
-
-
-
2.3.1.109 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.109
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
2.3.1.109 -20°C, retains full activity with both substrates for several weeks, loses 10-40% of its activity following repeated freezing and thawing Pseudomonas aeruginosa
2.3.1.109 4°C, 10 mM potassium phosphate, pH 7.5, stable for 48 h Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.109 additional information D-arginine, D-ornithine, L-lysine, L-citrulline, 2,4-aminobutyrate, 5-aminovalerate, 2-aminovalerate, agmatine or the polyamines propanediamine, putrescine, cadaverine and spermidine are not substrates Pseudomonas aeruginosa ?
-
 ?
2.3.1.109 succinyl-CoA + L-arginine
-
 Pseudomonas aeruginosa CoA + N2-succinyl-L-arginine
-
 ?
2.3.1.109 succinyl-CoA + L-homoarginine
-
 Pseudomonas aeruginosa CoA + N2-succinyl-L-homoarginine
-
 ?
2.3.1.109 succinyl-CoA + L-ornithine
-
 Pseudomonas aeruginosa CoA + N2-succinyl-L-ornithine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.109 tetramer 2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.109 8.5
-
 L-homoarginine as substrate Pseudomonas aeruginosa
2.3.1.109 8.7
-
 L-arginine as substrate Pseudomonas aeruginosa
2.3.1.109 9
-
 L-ornithine as substrate Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.109 0.9
-
 spermidine
-
 Pseudomonas aeruginosa
2.3.1.109 2.6
-
 D-arginine
-
 Pseudomonas aeruginosa
2.3.1.109 3.4
-
 agmatine
-
 Pseudomonas aeruginosa