BRENDA - Enzyme Database

The potential for platelet-activating factor synthesis in brain: properties of cholinephosphotransferase and 1-alkyl-sn-glycero-3-phosphate acetyltransferase in microsomal fractions of immature rabbit cerebral cortex

Baker, R.R.; Chang, H.Y.; Biochim. Biophys. Acta 1170, 157-164 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.105
1-alkyl-sn-glycero-3-phosphocholine
small increase of activity
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.105
dithiothreitol
-
Oryctolagus cuniculus
2.3.1.105
additional information
not inhibited by 1-alkyl-sn-glycero-3-phosphocholine, 1-alkyl-sn-glycero-3-phosphocholine
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.105
0.11
-
acetyl-CoA
apparent Km for subcellular fraction R
Oryctolagus cuniculus
2.3.1.105
0.137
-
acetyl-CoA
apparent Km for subcellular fraction P3
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.105
cell body
brain
Oryctolagus cuniculus
44297
-
2.3.1.105
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.105
Na+
activity appears maximal in the presence of NaF
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.105
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
Oryctolagus cuniculus
-
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
Oryctolagus cuniculus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.105
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.105
isolation of four subcellular fractions: S, R and P3 from cerebral cortices and P from nerve cell bodies
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.105
cerebral cortex
-
Oryctolagus cuniculus
-
2.3.1.105
nerve
brain
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.105
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
-
486105
Oryctolagus cuniculus
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
486105
Oryctolagus cuniculus
?
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.105
8
9
subcellular fractions R and P3
Oryctolagus cuniculus
2.3.1.105
8.4
9
subcellular fractions P
Oryctolagus cuniculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.105
1-alkyl-sn-glycero-3-phosphocholine
small increase of activity
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.105
dithiothreitol
-
Oryctolagus cuniculus
2.3.1.105
additional information
not inhibited by 1-alkyl-sn-glycero-3-phosphocholine, 1-alkyl-sn-glycero-3-phosphocholine
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.105
0.11
-
acetyl-CoA
apparent Km for subcellular fraction R
Oryctolagus cuniculus
2.3.1.105
0.137
-
acetyl-CoA
apparent Km for subcellular fraction P3
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.105
cell body
brain
Oryctolagus cuniculus
44297
-
2.3.1.105
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.105
Na+
activity appears maximal in the presence of NaF
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.105
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
Oryctolagus cuniculus
-
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.105
isolation of four subcellular fractions: S, R and P3 from cerebral cortices and P from nerve cell bodies
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.105
cerebral cortex
-
Oryctolagus cuniculus
-
2.3.1.105
nerve
brain
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.105
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphate
-
486105
Oryctolagus cuniculus
CoA + 1-alkyl-2-acetyl-sn-glycero-3-phosphate
-
486105
Oryctolagus cuniculus
?
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.105
8
9
subcellular fractions R and P3
Oryctolagus cuniculus
2.3.1.105
8.4
9
subcellular fractions P
Oryctolagus cuniculus