BRENDA - Enzyme Database

Isolation and properties of N epsilon-hydroxylysine:acetyl coenzyme A nepsilon-transacetylase from Escherichia coli pABN11

Coy, M.; Paw, B.H.; Bindereif, A.; Neilands, J.B.; Biochemistry 25, 2485-2489 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.102
Coomassie brilliant blue
mixed inhibitor
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.102
0.043
-
acetyl-CoA
+ L-N6-hydroxylysine
Escherichia coli
2.3.1.102
0.055
-
L-N6-hydroxylysine
-
Escherichia coli
2.3.1.102
0.079
-
acetyl-CoA
+ L-N5-hydroxyornithine
Escherichia coli
2.3.1.102
0.099
-
acetyl-CoA
+ 1-amino-5-(hydroxyamino)pentane
Escherichia coli
2.3.1.102
0.101
-
acetyl-CoA
+ N-methylhydroxylamine
Escherichia coli
2.3.1.102
0.317
-
L-N5-hydroxyornithine
-
Escherichia coli
2.3.1.102
0.33
-
1-amino-5-(hydroxyamino)pentane
-
Escherichia coli
2.3.1.102
0.396
-
N-Methylhydroxylamine
-
Escherichia coli
2.3.1.102
1.81
-
acetyl-CoA
+ hydroxylamine
Escherichia coli
2.3.1.102
329.4
-
hydroxylamine
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.102
33000
-
x * 33000, SDS-PAGE
Escherichia coli
2.3.1.102
150000
200000
gel filtration, native enzyme
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.102
additional information
Escherichia coli
enzyme is involved in the biosynthesis of aerobactin in E. coli
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.102
Escherichia coli
-
294, with plasmid pABN11
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.102
with plasmid pABN11
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.102
26
-
N6-hydroxylysine
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.102
20C, stable for several days
Escherichia coli
2.3.1.102
4C, stable for several months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.102
acetyl-CoA + (5-hydroxyamine)pentan-1-amine
-
486098
Escherichia coli
CoA + N-(5-aminopentyl)-N-hydroxyacetamide
-
-
-
?
2.3.1.102
acetyl-CoA + DL-N6-hydroxylysine
-
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
-
-
?
2.3.1.102
acetyl-CoA + hydroxylamine
-
486098
Escherichia coli
CoA + N-acetylhydroxylamine
-
-
-
?
2.3.1.102
acetyl-CoA + L-N5-hydroxyornithine
-
486098
Escherichia coli
CoA + N5-acetyl-N5-hydroxyornithine
-
-
-
?
2.3.1.102
acetyl-CoA + L-N6-hydroxylysine
preferred substrate
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
486098
Escherichia coli
?
2.3.1.102
acetyl-CoA + N-methylhydroxylamine
-
486098
Escherichia coli
CoA + N-acetyl-N-methylhydroxylamine
-
-
-
?
2.3.1.102
additional information
propionyl-CoA has 5% of activity compared to acetyl-CoA
486098
Escherichia coli
?
-
-
-
-
2.3.1.102
additional information
enzyme is involved in the biosynthesis of aerobactin in E. coli
486098
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.102
?
x * 33000, SDS-PAGE
Escherichia coli
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.102
40
-
sharp decline above
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.102
0.0000385
-
N-Methylhydroxylamine
-
Escherichia coli
2.3.1.102
0.0000477
-
N6-hydroxylysine
-
Escherichia coli
2.3.1.102
0.0000797
-
1-amino-5(hydroxyamino)pentane
-
Escherichia coli
2.3.1.102
0.000112
-
N6-Hydroxyornithine
-
Escherichia coli
2.3.1.102
0.00177
-
hydroxylamine
-
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.102
0.00071
-
Coomassie brilliant blue
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.102
Coomassie brilliant blue
mixed inhibitor
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.102
0.00071
-
Coomassie brilliant blue
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.102
0.043
-
acetyl-CoA
+ L-N6-hydroxylysine
Escherichia coli
2.3.1.102
0.055
-
L-N6-hydroxylysine
-
Escherichia coli
2.3.1.102
0.079
-
acetyl-CoA
+ L-N5-hydroxyornithine
Escherichia coli
2.3.1.102
0.099
-
acetyl-CoA
+ 1-amino-5-(hydroxyamino)pentane
Escherichia coli
2.3.1.102
0.101
-
acetyl-CoA
+ N-methylhydroxylamine
Escherichia coli
2.3.1.102
0.317
-
L-N5-hydroxyornithine
-
Escherichia coli
2.3.1.102
0.33
-
1-amino-5-(hydroxyamino)pentane
-
Escherichia coli
2.3.1.102
0.396
-
N-Methylhydroxylamine
-
Escherichia coli
2.3.1.102
1.81
-
acetyl-CoA
+ hydroxylamine
Escherichia coli
2.3.1.102
329.4
-
hydroxylamine
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.102
33000
-
x * 33000, SDS-PAGE
Escherichia coli
2.3.1.102
150000
200000
gel filtration, native enzyme
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.102
additional information
Escherichia coli
enzyme is involved in the biosynthesis of aerobactin in E. coli
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.102
with plasmid pABN11
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.102
26
-
N6-hydroxylysine
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.102
20C, stable for several days
Escherichia coli
2.3.1.102
4C, stable for several months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.102
acetyl-CoA + (5-hydroxyamine)pentan-1-amine
-
486098
Escherichia coli
CoA + N-(5-aminopentyl)-N-hydroxyacetamide
-
-
-
?
2.3.1.102
acetyl-CoA + DL-N6-hydroxylysine
-
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
-
-
?
2.3.1.102
acetyl-CoA + hydroxylamine
-
486098
Escherichia coli
CoA + N-acetylhydroxylamine
-
-
-
?
2.3.1.102
acetyl-CoA + L-N5-hydroxyornithine
-
486098
Escherichia coli
CoA + N5-acetyl-N5-hydroxyornithine
-
-
-
?
2.3.1.102
acetyl-CoA + L-N6-hydroxylysine
preferred substrate
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
486098
Escherichia coli
?
2.3.1.102
acetyl-CoA + N-methylhydroxylamine
-
486098
Escherichia coli
CoA + N-acetyl-N-methylhydroxylamine
-
-
-
?
2.3.1.102
additional information
propionyl-CoA has 5% of activity compared to acetyl-CoA
486098
Escherichia coli
?
-
-
-
-
2.3.1.102
additional information
enzyme is involved in the biosynthesis of aerobactin in E. coli
486098
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.102
?
x * 33000, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.102
40
-
sharp decline above
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.102
0.0000385
-
N-Methylhydroxylamine
-
Escherichia coli
2.3.1.102
0.0000477
-
N6-hydroxylysine
-
Escherichia coli
2.3.1.102
0.0000797
-
1-amino-5(hydroxyamino)pentane
-
Escherichia coli
2.3.1.102
0.000112
-
N6-Hydroxyornithine
-
Escherichia coli
2.3.1.102
0.00177
-
hydroxylamine
-
Escherichia coli