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Literature summary extracted from

  • Coy, M.; Paw, B.H.; Bindereif, A.; Neilands, J.B.
    Isolation and properties of N epsilon-hydroxylysine:acetyl coenzyme A nepsilon-transacetylase from Escherichia coli pABN11 (1986), Biochemistry, 25, 2485-2489.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.102 Coomassie brilliant blue mixed inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.102 0.043
-
 acetyl-CoA + L-N6-hydroxylysine Escherichia coli
2.3.1.102 0.055
-
 L-N6-hydroxylysine
-
 Escherichia coli
2.3.1.102 0.079
-
 acetyl-CoA + L-N5-hydroxyornithine Escherichia coli
2.3.1.102 0.099
-
 acetyl-CoA + 1-amino-5-(hydroxyamino)pentane Escherichia coli
2.3.1.102 0.101
-
 acetyl-CoA + N-methylhydroxylamine Escherichia coli
2.3.1.102 0.317
-
 L-N5-hydroxyornithine
-
 Escherichia coli
2.3.1.102 0.33
-
 1-amino-5-(hydroxyamino)pentane
-
 Escherichia coli
2.3.1.102 0.396
-
 N-Methylhydroxylamine
-
 Escherichia coli
2.3.1.102 1.81
-
 acetyl-CoA + hydroxylamine Escherichia coli
2.3.1.102 329.4
-
 hydroxylamine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.102 33000
-
 x * 33000, SDS-PAGE Escherichia coli
2.3.1.102 150000 200000 gel filtration, native enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.102 additional information Escherichia coli enzyme is involved in the biosynthesis of aerobactin in E. coli ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.102 Escherichia coli
-
 294, with plasmid pABN11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.102 with plasmid pABN11 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.102 26
-
 N6-hydroxylysine Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.102 20°C, stable for several days Escherichia coli
2.3.1.102 4°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.102 acetyl-CoA + (5-hydroxyamine)pentan-1-amine
-
 Escherichia coli CoA + N-(5-aminopentyl)-N-hydroxyacetamide
-
 ?
2.3.1.102 acetyl-CoA + DL-N6-hydroxylysine
-
 Escherichia coli CoA + N6-acetyl-N6-hydroxylysine
-
 ?
2.3.1.102 acetyl-CoA + hydroxylamine
-
 Escherichia coli CoA + N-acetylhydroxylamine
-
 ?
2.3.1.102 acetyl-CoA + L-N5-hydroxyornithine
-
 Escherichia coli CoA + N5-acetyl-N5-hydroxyornithine
-
 ?
2.3.1.102 acetyl-CoA + L-N6-hydroxylysine preferred substrate Escherichia coli CoA + N6-acetyl-N6-hydroxylysine
-
 ?
2.3.1.102 acetyl-CoA + N-methylhydroxylamine
-
 Escherichia coli CoA + N-acetyl-N-methylhydroxylamine
-
 ?
2.3.1.102 acetyl-CoA + N5-hydroxyornithine
-
 Escherichia coli CoA + N5-acetyl-N5-hydroxyornithine
-
 r
2.3.1.102 additional information propionyl-CoA has 5% of activity compared to acetyl-CoA Escherichia coli ?
-
 ?
2.3.1.102 additional information enzyme is involved in the biosynthesis of aerobactin in E. coli Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.102 ? x * 33000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.102 40
-
 sharp decline above Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.102 0.0000385
-
 N-Methylhydroxylamine
-
 Escherichia coli
2.3.1.102 0.0000477
-
 N6-hydroxylysine
-
 Escherichia coli
2.3.1.102 0.0000797
-
 1-amino-5(hydroxyamino)pentane
-
 Escherichia coli
2.3.1.102 0.000112
-
 N5-hydroxyornithine
-
 Escherichia coli
2.3.1.102 0.00177
-
 hydroxylamine
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.102 0.00071
-
 Coomassie brilliant blue
-
 Escherichia coli