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Literature summary extracted from

  • Bizzozero, O.A.; McGarry, J.F.; Lees, M.B.
    Acylation of endogenous myelin proteolipid protein with different acyl-CoAs (1987), J. Biol. Chem., 262, 2138-2145.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.100 dithiothreitol 1 mM, slightly stimulates Rattus norvegicus
2.3.1.100 Nonidet P-40 stimulates at 0.05% Rattus norvegicus
2.3.1.100 octyl glucoside slight stimulation Rattus norvegicus
2.3.1.100 sodium deoxycholate stimulates at 0.5% Rattus norvegicus
2.3.1.100 Triton X-100 0.1%, stimulates Rattus norvegicus
2.3.1.100 Tween 20 0.05%, slight stimulation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.100 Sodium dodecyl sulfate at concentration 0.5%: 92% loss of activity Rattus norvegicus
2.3.1.100 Tween 20 inhibits slightly at 0.5% Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.100 0.041
-
 palmitoyl-CoA
-
 Rattus norvegicus
2.3.1.100 0.046
-
 oleoyl-CoA
-
 Rattus norvegicus
2.3.1.100 0.05
-
 stearoyl-CoA
-
 Rattus norvegicus
2.3.1.100 0.219
-
 myristoyl-CoA
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.100 MgCl2 2 mM, stimulates Rattus norvegicus
2.3.1.100 MnCl2 stimulates Rattus norvegicus
2.3.1.100 NaF slight stimulation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.100 additional information Rattus norvegicus enzyme is probably involved in the acylation of endogenous myelin proteolipid protein ?
-
 ?
2.3.1.100 palmitoyl-CoA + myelin proteolipid protein Rattus norvegicus
-
 CoA + myelin proteolipid O-palmitoylprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.100 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.100 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.100 brain myelin labeled product also found in vitro in brain tissue slices Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.100 freezing and thawing causes marked decrease in activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.100 additional information enzyme is probably involved in the acylation of endogenous myelin proteolipid protein Rattus norvegicus ?
-
 ?
2.3.1.100 myristoyl-CoA + myelin proteolipid protein low activity Rattus norvegicus CoA + myelin proteolipid O-myristoylprotein
-
 ?
2.3.1.100 oleoyl-CoA + myelin proteolipid protein
-
 Rattus norvegicus CoA + myelin proteolipid O-oleoylprotein
-
 ?
2.3.1.100 palmitoyl-CoA + myelin proteolipid protein
-
 Rattus norvegicus CoA + myelin proteolipid O-palmitoylprotein
-
 ?
2.3.1.100 stearoyl-CoA + myelin proteolipid protein
-
 Rattus norvegicus CoA + myelin proteolipid O-stearoylprotein
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.100 37
-
 assay at Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.100 10 50 45% of maximal acticity at 10°C, 75% of maximal activity at 50°C Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.100 75
-
 5 min, complete loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.100 7.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.100 6 8 25% of maximal activity at pH 6, 90% of maximal activity at pH 8 Rattus norvegicus