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Literature summary extracted from

  • Waites, M.J.; Quayle, J.R.
    Dihydroxyacetone synthase: a special transketolase for formaldehyde fixation from the methylotrophic yeast Candida boidinii CBS 5777 (1983), J. Gen. Microbiol., 129, 935-944.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.3 Co2+
-
 [Candida] boidinii
2.2.1.3 GSH
-
 [Candida] boidinii
2.2.1.3 Ni2+
-
 [Candida] boidinii
2.2.1.3 ribose
-
 [Candida] boidinii
2.2.1.3 sulfate
-
 [Candida] boidinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.3 Mg2+ requirement [Candida] boidinii
2.2.1.3 Mn2+ activation, replaces Mg2+ to some extent [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.3 62500
-
 alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE [Candida] boidinii
2.2.1.3 65500
-
 alpha2, 2 * 65500, SDS-PAGE [Candida] boidinii
2.2.1.3 105000 110000 gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde [Candida] boidinii
-
 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.3 [Candida] boidinii
-
 methanol grown
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.3 0.766
-
-
 [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + butyraldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + 1,3-dihydroxyhexane-2-one
-
 ?
2.2.1.3 D-xylulose 5-phosphate + D-erythrose
-
 [Candida] boidinii glyceraldehyde 3-phosphate + D-fructose
-
 ?
2.2.1.3 D-xylulose 5-phosphate + D-glucose
-
 [Candida] boidinii glyceraldehyde 3-phosphate + D-glycero-D-ido-oct-2-ulose
-
 ?
2.2.1.3 D-xylulose 5-phosphate + D-ribose
-
 [Candida] boidinii glyceraldehyde 3-phosphate + sedoheptulose
-
 ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde best substrate [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde D-xylulose 5-phosphate cannot be replaced by D-ribulose 5-phosphate, D-glucose 6-phosphate, or D-ribose 5-phosphate [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + formaldehyde no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 D-xylulose 5-phosphate + glycolaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + 1,3,5-trihydroxypentane-2-one
-
 ?
2.2.1.3 D-xylulose 5-phosphate + heptaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + 1,3-dihydroxynonane-2-one
-
 ?
2.2.1.3 D-xylulose 5-phosphate + propionaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + 1,3-dihydroxypentane-2-one
-
 ?
2.2.1.3 fructose 6-phosphate + formaldehyde fructose 6-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor [Candida] boidinii dihydroxyacetone + erythrose 4-phosphate
-
 ?
2.2.1.3 hydroxypyruvate + formaldehyde hydroxypyruvate can replace D-xylulose 5-phosphate with 41.4%, hydroxypyruvate can replace D-xylulose 5-phosphate with 56% [Candida] boidinii CO2 + dihydroxyacetone
-
 ?
2.2.1.3 sedoheptulose 7-phosphate + formaldehyde sedoheptulose 7-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor [Candida] boidinii dihydroxyacetone + ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.3 dimer alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE [Candida] boidinii
2.2.1.3 dimer alpha2, 2 * 65500, SDS-PAGE [Candida] boidinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.3 35
-
 15 min in stabilizing buffer [Candida] boidinii
2.2.1.3 35
-
 5 min without stabilizing agents [Candida] boidinii