BRENDA - Enzyme Database

Properties and functions of the thiamin diphosphate dependent enzyme transketolase

Schenk, G.; Duggleby, R.G.; Nixon, P.F.; Int. J. Biochem. Cell Biol. 30, 1297-1318 (1998)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.2.1.1
freezing and thawing causes loss of activity
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2.1.1
0.09
-
D-erythrose 4-phosphate
-
Escherichia coli
2.2.1.1
0.16
-
D-xylulose 5-phosphate
-
Escherichia coli
2.2.1.1
0.21
-
D-xylulose 5-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
0.36
-
D-erythrose 4-phosphate
-
Homo sapiens
2.2.1.1
0.4
-
D-ribose 5-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
0.4
-
D-ribose 5-phosphate
-
Spinacia oleracea
2.2.1.1
0.49
-
D-xylulose 5-phosphate
-
Homo sapiens
2.2.1.1
0.53
-
D-ribose 5-phosphate
-
Homo sapiens
2.2.1.1
1.1
-
fructose 6-phosphate
-
Escherichia coli
2.2.1.1
1.4
-
D-ribose 5-phosphate
-
Escherichia coli
2.2.1.1
1.8
-
fructose 6-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
2.1
-
D,L-glyceraldehyde 3-phosphate
-
Escherichia coli
2.2.1.1
3.2
-
fructose 6-phosphate
-
Spinacia oleracea
2.2.1.1
4.9
-
glyceraldehyde 3-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
7
-
fructose 6-phosphate
-
Homo sapiens
2.2.1.1
18
-
Hydroxypyruvate
-
Escherichia coli
2.2.1.1
33
-
Hydroxypyruvate
-
Saccharomyces cerevisiae
2.2.1.1
45
-
D-xylose
-
Spinacia oleracea
2.2.1.1
55
-
L-arabinose
-
Spinacia oleracea
2.2.1.1
120
-
L-Lyxose
-
Spinacia oleracea
2.2.1.1
230
-
D-ribose
-
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.2.1.1
Cyberlindnera jadinii
-
-
-
2.2.1.1
Escherichia coli
-
-
-
2.2.1.1
Homo sapiens
-
-
-
2.2.1.1
Mus musculus
-
-
-
2.2.1.1
Oryctolagus cuniculus
-
-
-
2.2.1.1
Rattus norvegicus
-
-
-
2.2.1.1
Saccharomyces cerevisiae
P23254
baker's yeast
-
2.2.1.1
Saccharomyces pastorianus
-
-
-
2.2.1.1
Spinacia oleracea
-
spinach
-
2.2.1.1
Sus scrofa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.2.1.1
brain
-
Mus musculus
-
2.2.1.1
erythrocyte
-
Homo sapiens
-
2.2.1.1
leukocyte
-
Homo sapiens
-
2.2.1.1
liver
-
Oryctolagus cuniculus
-
2.2.1.1
liver
-
Rattus norvegicus
-
2.2.1.1
liver
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.1
D-erythrose 4-phosphate + ?
-
486026
Escherichia coli
?
-
-
-
?
2.2.1.1
D-erythrose 4-phosphate + ?
-
486026
Homo sapiens
?
-
-
-
?
2.2.1.1
D-ribose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
D-xylose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Saccharomyces pastorianus
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Cyberlindnera jadinii
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Escherichia coli
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Homo sapiens
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Rattus norvegicus
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Sus scrofa
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Saccharomyces cerevisiae
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Oryctolagus cuniculus
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Spinacia oleracea
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
DL-glyceraldehyde 3-phosphate + ?
-
486026
Escherichia coli
?
-
-
-
?
2.2.1.1
DL-glyceraldehyde 3-phosphate + ?
-
486026
Saccharomyces cerevisiae
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Escherichia coli
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Homo sapiens
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Saccharomyces cerevisiae
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
hydroxypyruvate + ribose 5-phosphate
-
486026
Escherichia coli
sedoheptulose 7-phosphate + ?
-
-
-
?
2.2.1.1
hydroxypyruvate + ribose 5-phosphate
-
486026
Saccharomyces cerevisiae
sedoheptulose 7-phosphate + ?
-
-
-
?
2.2.1.1
L-arabinose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
L-lyxose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.1
7
7.5
in phosphate buffer
Escherichia coli
2.2.1.1
7.5
7.6
-
Saccharomyces cerevisiae
2.2.1.1
7.5
7.6
-
Saccharomyces pastorianus
2.2.1.1
7.5
7.6
-
Spinacia oleracea
2.2.1.1
7.7
-
-
Cyberlindnera jadinii
2.2.1.1
7.7
-
-
Homo sapiens
2.2.1.1
8
8.5
in glycylglycine buffer
Escherichia coli
2.2.1.1
8
8.5
-
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
2.2.1.1
freezing and thawing causes loss of activity
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2.1.1
0.09
-
D-erythrose 4-phosphate
-
Escherichia coli
2.2.1.1
0.16
-
D-xylulose 5-phosphate
-
Escherichia coli
2.2.1.1
0.21
-
D-xylulose 5-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
0.36
-
D-erythrose 4-phosphate
-
Homo sapiens
2.2.1.1
0.4
-
D-ribose 5-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
0.4
-
D-ribose 5-phosphate
-
Spinacia oleracea
2.2.1.1
0.49
-
D-xylulose 5-phosphate
-
Homo sapiens
2.2.1.1
0.53
-
D-ribose 5-phosphate
-
Homo sapiens
2.2.1.1
1.1
-
fructose 6-phosphate
-
Escherichia coli
2.2.1.1
1.4
-
D-ribose 5-phosphate
-
Escherichia coli
2.2.1.1
1.8
-
fructose 6-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
2.1
-
D,L-glyceraldehyde 3-phosphate
-
Escherichia coli
2.2.1.1
3.2
-
fructose 6-phosphate
-
Spinacia oleracea
2.2.1.1
4.9
-
glyceraldehyde 3-phosphate
-
Saccharomyces cerevisiae
2.2.1.1
7
-
fructose 6-phosphate
-
Homo sapiens
2.2.1.1
18
-
Hydroxypyruvate
-
Escherichia coli
2.2.1.1
33
-
Hydroxypyruvate
-
Saccharomyces cerevisiae
2.2.1.1
45
-
D-xylose
-
Spinacia oleracea
2.2.1.1
55
-
L-arabinose
-
Spinacia oleracea
2.2.1.1
120
-
L-Lyxose
-
Spinacia oleracea
2.2.1.1
230
-
D-ribose
-
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.2.1.1
brain
-
Mus musculus
-
2.2.1.1
erythrocyte
-
Homo sapiens
-
2.2.1.1
leukocyte
-
Homo sapiens
-
2.2.1.1
liver
-
Oryctolagus cuniculus
-
2.2.1.1
liver
-
Rattus norvegicus
-
2.2.1.1
liver
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.1
D-erythrose 4-phosphate + ?
-
486026
Escherichia coli
?
-
-
-
?
2.2.1.1
D-erythrose 4-phosphate + ?
-
486026
Homo sapiens
?
-
-
-
?
2.2.1.1
D-ribose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
D-xylose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Saccharomyces pastorianus
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Cyberlindnera jadinii
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Escherichia coli
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Homo sapiens
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Rattus norvegicus
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Sus scrofa
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Saccharomyces cerevisiae
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Oryctolagus cuniculus
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
486026
Spinacia oleracea
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
-
-
r
2.2.1.1
DL-glyceraldehyde 3-phosphate + ?
-
486026
Escherichia coli
?
-
-
-
?
2.2.1.1
DL-glyceraldehyde 3-phosphate + ?
-
486026
Saccharomyces cerevisiae
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Escherichia coli
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Homo sapiens
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Saccharomyces cerevisiae
?
-
-
-
?
2.2.1.1
fructose 6-phosphate + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
hydroxypyruvate + ribose 5-phosphate
-
486026
Escherichia coli
sedoheptulose 7-phosphate + ?
-
-
-
?
2.2.1.1
hydroxypyruvate + ribose 5-phosphate
-
486026
Saccharomyces cerevisiae
sedoheptulose 7-phosphate + ?
-
-
-
?
2.2.1.1
L-arabinose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
2.2.1.1
L-lyxose + ?
-
486026
Spinacia oleracea
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.1
7
7.5
in phosphate buffer
Escherichia coli
2.2.1.1
7.5
7.6
-
Saccharomyces cerevisiae
2.2.1.1
7.5
7.6
-
Saccharomyces pastorianus
2.2.1.1
7.5
7.6
-
Spinacia oleracea
2.2.1.1
7.7
-
-
Cyberlindnera jadinii
2.2.1.1
7.7
-
-
Homo sapiens
2.2.1.1
8
8.5
in glycylglycine buffer
Escherichia coli
2.2.1.1
8
8.5
-
Homo sapiens