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Literature summary extracted from

  • Wikner, C.; Nilsson, U.; Meshalkina, L.; Udekwu, C.; Lindqvist, Y.; Schneider, G.
    Identification of catalytically important residues in yeast transketolase (1997), Biochemistry, 36, 15643-15649.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 H263A lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 H30A lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 H30N lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 H481A lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 H481Q lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 H481S lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 H69A lower activity than wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.0003
-
 thiamine diphosphate H481A Saccharomyces cerevisiae
2.2.1.1 0.0005
-
 thiamine diphosphate H30N, H418S Saccharomyces cerevisiae
2.2.1.1 0.0006
-
 thiamine diphosphate wild-type Saccharomyces cerevisiae
2.2.1.1 0.0007
-
 thiamine diphosphate H481Q Saccharomyces cerevisiae
2.2.1.1 0.0008
-
 thiamine diphosphate H30A Saccharomyces cerevisiae
2.2.1.1 0.0012
-
 thiamine diphosphate H263A Saccharomyces cerevisiae
2.2.1.1 0.0018
-
 thiamine diphosphate H69A Saccharomyces cerevisiae
2.2.1.1 0.023
-
 D-xylulose 5-phosphate H263A Saccharomyces cerevisiae
2.2.1.1 0.07
-
 D-xylulose 5-phosphate wild-type Saccharomyces cerevisiae
2.2.1.1 0.093
-
 D-ribose 5-phosphate H263A Saccharomyces cerevisiae
2.2.1.1 0.146
-
 D-ribose 5-phosphate wild-type Saccharomyces cerevisiae
2.2.1.1 0.15
-
 D-ribose 5-phosphate H481A Saccharomyces cerevisiae
2.2.1.1 0.193
-
 D-ribose 5-phosphate H69A Saccharomyces cerevisiae
2.2.1.1 0.29
-
 D-ribose 5-phosphate H30A Saccharomyces cerevisiae
2.2.1.1 0.4
-
 D-xylulose 5-phosphate H69A Saccharomyces cerevisiae
2.2.1.1 1.01
-
 D-xylulose 5-phosphate H30A Saccharomyces cerevisiae
2.2.1.1 1.24
-
 D-xylulose 5-phosphate H481A Saccharomyces cerevisiae
2.2.1.1 3.35
-
 D-xylulose 5-phosphate H481S Saccharomyces cerevisiae
2.2.1.1 4.08
-
 D-xylulose 5-phosphate H481Q Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Saccharomyces cerevisiae
-
 baker's yeast
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 6.6 7.4 H481Q Saccharomyces cerevisiae
2.2.1.1 6.8 8 H30A Saccharomyces cerevisiae
2.2.1.1 7 7.8 H481A Saccharomyces cerevisiae
2.2.1.1 7.5 7.8 H69A Saccharomyces cerevisiae
2.2.1.1 7.6 8.2 wild-type Saccharomyces cerevisiae
2.2.1.1 7.6 8 H263A Saccharomyces cerevisiae